JBIC Journal of Biological Inorganic Chemistry ( IF 2.7 ) Pub Date : 2021-08-28 , DOI: 10.1007/s00775-021-01888-2 Marcos V Palmeira-Mello 1, 2, 3 , Ana B Caballero 2 , Aida Lopez-Espinar 2 , Guilherme P Guedes 1 , Amparo Caubet 2 , Alessandra M Teles de Souza 3 , Mauricio Lanznaster 1 , Patrick Gamez 2, 4, 5
|
Two square-planar coordination compounds, namely [Cu(CPYA)Cl2] (1) and [Pd(CPYA)Cl2] (2), were prepared from the ligand 4-chloro-N-(pyridin-2-ylmethyl)aniline (CPYA) and two chloride salts, and were fully characterized, including by X-ray diffraction. Spectroscopic, electrophoretic and AFM studies revealed that the two isostructural compounds were interacting differently with DNA. In both cases, the initial interaction involves electrostatic contacts of the CPYA ligand in the minor groove (as suggested by molecular docking), but subsequent strong binding occurs with the palladium(II) complex 2, whereas the binding with the copper complex 1 is weaker and concentration dependent. The strong binding of 2 eventually leads to the cleavage of the double strand and the redox activity of 1 allows to oxidatively cleave the biomolecule.
Graphic abstract
中文翻译：
两种类似的方形平面顺氯络合物的 DNA 相互作用特性：铜与钯
以配体4-氯-N- (吡啶-2-基甲基)为原料制备了两种平面方形配位化合物，即[Cu(CPYA)Cl 2 ] ( 1 )和[Pd(CPYA)Cl 2 ] ( 2 )。苯胺 (CPYA) 和两种氯化物盐，并通过 X 射线衍射等方法进行了充分表征。光谱、电泳和 AFM 研究表明，这两种同构化合物与 DNA 的相互作用不同。在这两种情况下，最初的相互作用涉及小沟中 CPYA 配体的静电接触（如分子对接所示），但随后与钯（II）络合物2发生强结合，而与铜络合物1的结合较弱和浓度依赖性。 2的强结合最终导致双链裂解， 1的氧化还原活性允许氧化裂解生物分子。
京公网安备 11010802027423号