Stachybotrys (S.) chartarum had been related to dangerous health problems in animals and humans that take place when exposure to S. chartarum toxins. S. chartarum had been isolated from various substrates, ranging from inappropriately stored feed and culinary herbs to damp buildings. To evaluate the pathogenic potential of isolates, it is essential to identify them with different methods. The occurrence and genetic diversity of S. chartarum isolates from faba beans dust during threshing in Upper Egypt were investigated. Low counts of Stachybotrys were found (six isolates) and identified morphologically by single-spore isolation and molecularly by the amplification of the specific internal transcribed spacer (ITS) region and glyceraldehydes-3-phosphate dehydrogenase (gpd). The genetic diversity of the collected isolates was studied by specific genes random primer polymerase chain reaction (SGRP-PCR). The phylogenetic analysis of S. chartarum showed that the specific primers IT51 and StacR3 used by commercial laboratories for detecting S. chartarum were not able to differentiate species of S. chartarum from S. chlorohalonata and unweighted pair group method of arithmetic averages (UPGMA) cluster analysis of SGRP fragments confirmed this result. The six isolates of S. chartarum were analyzed for the presence of trichodiene synthase (Tri5) gene, which needed in the early stage of the trichothecene synthesis path. All the tested isolates were positive for the Tri5 gene. Further study on the taxonomic status of the epithet S. chartarum is necessary and presence of sub species to S. chartarum might be acceptable depending on the variations of morphological characteristics which were confirmed by molecular techniques.

Stachybotrys  ( S. )  chartarum 与动物和人类接触S时发生的危险健康问题有关。 图表毒素。 S. chartarum 已从各种基质中分离出来，从不当储存的饲料和烹饪草药到潮湿的建筑物。为了评估分离株的致病潜力，必须用不同的方法识别它们。调查了上埃及脱粒过程中蚕豆粉尘中S.chartarum分离株的发生情况和遗传多样性 。低计数葡萄穗被发现（六个分离株）并通过单孢子分离进行形态学鉴定，并通过特定内部转录间隔区 (ITS) 区域和甘油醛-3-磷酸脱氢酶 (gpd) 的扩增进行分子鉴定。通过特定基因随机引物聚合酶链反应（SGRP-PCR）研究收集到的分离株的遗传多样性。S. chartarum的系统发育分析表明，商业实验室用于检测S. chartarum的特异性引物 IT51 和 StacR3无法区分S. chartarum与S. chlorohalonata和算术平均值的未加权配对组方法 (UPGMA) 聚类SGRP 片段的分析证实了这一结果。六株分离株分析了S. chartarum是否存在单端孢菌素合成酶 ( Tri5 ) 基因，该基因在单端孢菌素合成路径的早期阶段需要。所有受试分离株的Tri5基因均呈阳性。进一步研究加词S. chartarum的分类地位是必要的，根据分子技术证实的形态特征的变化，S. chartarum亚种的存在可能是可以接受的。