We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.

中文翻译：

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血液子孢子和人类疟疾物种子孢子的流式细胞术和 ImageStream Rna-Fish 基因表达、定量和表型特征

我们采用 RNA FISH Stellaris 方法，通过流式细胞术和 ImageStream (Flow-FISH) 特异性检测疟原虫基因的表达。这种新方法准确地量化了患者分离株中的（1）恶性疟原虫和间日疟原虫的红细胞形式，以及（2）间日疟原虫的有性阶段。使用表面环子孢子蛋白 (CSP)、脱氧核糖核酸和 18S RNA 标记组合对肝脏阶段子孢子进行 ImageStream 分析，证明新的 Flow-FISH 适用于传播阶段的基因表达研究。这种强大的多参数单细胞方法为疟原虫生物学的应用和基础研究提供了一个选择平台。