Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications,JBIC Journal of Biological Inorganic Chemistry

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Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications
JBIC Journal of Biological Inorganic Chemistry ( IF 2.7 ) Pub Date : 2021-08-27 , DOI: 10.1007/s00775-021-01886-4
Francisco Romero-Gavilán ₁ , Andreia Cerqueira ₁ , Eduardo Anitua _{2,

3,

4} , Ricardo Tejero _{2,

3} , Iñaki García-Arnáez ₅ , Cristina Martinez-Ramos ₆ , Seda Ozturan ₇ , Raul Izquierdo ₁ , Mikel Azkargorta ₈ , Félix Elortza ₈ , Mariló Gurruchaga ₅ , Isabel Goñi ₅ , Julio Suay ₁

Affiliation

Calcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant–tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant–blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces.

Graphic abstract

中文翻译：

富含钙的钛表面上的蛋白质吸附/解吸动力学：生物学意义

钙离子用于开发生物材料以促进凝血、骨再生和植入物骨整合。植入后，钛植入物表面钙离子的时间依赖性释放改变了植入物-组织界面的物理化学特性，从而改变了生物反应。本研究的目的是研究这些表面上蛋白质吸附的动态如何随时间变化。将含有和不含 Ca 的钛圆盘与人血清一起孵育 2 分钟、180 分钟和 960 分钟。使用 nLC-MS/MS 对附着在表面的蛋白质层进行表征。材料之间的吸附动力学是不同的，表明在 Ca 释放之前与凝血和免疫反应相关的蛋白质的吸附增加。植入物-血液接触实验证实了对 Ca 表面的强凝血作用。我们采用原代人肺泡成骨细胞和 THP-1 单核细胞来研究成骨和炎症反应。与蛋白质组学结果一致，富含 Ca 的表面显示出明显的初始炎症，一旦钙被释放，炎症就会消失。在这项工作中发现的独特的蛋白质吸附/解吸动力学证明有助于解释钛和钙离子改性植入物表面之间的不同生物反应。富含 Ca 的表面显示出明显的初始炎症，一旦钙被释放，炎症就会消失。在这项工作中发现的独特的蛋白质吸附/解吸动力学证明有助于解释钛和钙离子改性植入物表面之间的不同生物反应。富含 Ca 的表面显示出明显的初始炎症，一旦钙被释放，炎症就会消失。在这项工作中发现的独特的蛋白质吸附/解吸动力学证明有助于解释钛和钙离子改性植入物表面之间的不同生物反应。

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更新日期：2021-08-29

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