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Light-Triggered Release of Large Biomacromolecules from Porphyrin-Phospholipid Liposomes
Langmuir ( IF 3.7 ) Pub Date : 2021-08-27 , DOI: 10.1021/acs.langmuir.1c01848 Hailey I Kilian 1 , Apoorva J Pradhan 2 , Dushyant Jahagirdar 3 , Joaquin Ortega 3 , Gunes Ekin Atilla-Gokcumen 2 , Jonathan F Lovell 1
Langmuir ( IF 3.7 ) Pub Date : 2021-08-27 , DOI: 10.1021/acs.langmuir.1c01848 Hailey I Kilian 1 , Apoorva J Pradhan 2 , Dushyant Jahagirdar 3 , Joaquin Ortega 3 , Gunes Ekin Atilla-Gokcumen 2 , Jonathan F Lovell 1
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Liposomes containing small amounts of porphyrin-phospholipid (PoP) have been shown to encapsulate small molecular weight cargos and then release them upon exposure to red light. A putative mechanism involves transient pore formation in the bilayer induced by PoP-mediated photo-oxidation of unsaturated lipids. However, little is known about the properties of such pores. This study assesses whether large carbohydrate and protein molecules could be released from PoP liposomes upon red light exposure. A small fluorophore with ∼0.5 kDa in molecular weight, fluorescently labeled dextrans of ∼5 and ∼500 kDa, and a ∼240 kDa fluorescent protein were passively entrapped in PoP liposomes. When exposed to 665 nm irradiation, liposomes containing PoP, but not liposomes lacking it, released all these cargos in a size-dependent manner that occurred with oxidation of unsaturated lipids included in the bilayer. Thus, this study demonstrates the feasibility of light-triggered release of large biomacromolecules from liposomes.
中文翻译：
从卟啉-磷脂脂质体中光触发释放大生物大分子
含有少量卟啉-磷脂 (PoP) 的脂质体已被证明可以封装小分子量的货物，然后在暴露于红光时将其释放。一种假定的机制涉及由 PoP 介导的不饱和脂质光氧化诱导的双层中的瞬时孔形成。然而，人们对这种孔隙的特性知之甚少。该研究评估了在红光照射下是否可以从 PoP 脂质体中释放大的碳水化合物和蛋白质分子。分子量约 0.5 kDa 的小荧光团、约 5 和约 500 kDa 的荧光标记葡聚糖以及约 240 kDa 的荧光蛋白被被动捕获在 PoP 脂质体中。当暴露于 665 nm 辐射时，含有 PoP 的脂质体，而不是缺乏它的脂质体，以大小依赖的方式释放所有这些货物，这种方式发生在双层中不饱和脂质的氧化过程中。因此，这项研究证明了从脂质体中光触发释放大生物大分子的可行性。
更新日期：2021-09-14
中文翻译：
从卟啉-磷脂脂质体中光触发释放大生物大分子
含有少量卟啉-磷脂 (PoP) 的脂质体已被证明可以封装小分子量的货物，然后在暴露于红光时将其释放。一种假定的机制涉及由 PoP 介导的不饱和脂质光氧化诱导的双层中的瞬时孔形成。然而，人们对这种孔隙的特性知之甚少。该研究评估了在红光照射下是否可以从 PoP 脂质体中释放大的碳水化合物和蛋白质分子。分子量约 0.5 kDa 的小荧光团、约 5 和约 500 kDa 的荧光标记葡聚糖以及约 240 kDa 的荧光蛋白被被动捕获在 PoP 脂质体中。当暴露于 665 nm 辐射时，含有 PoP 的脂质体，而不是缺乏它的脂质体，以大小依赖的方式释放所有这些货物，这种方式发生在双层中不饱和脂质的氧化过程中。因此，这项研究证明了从脂质体中光触发释放大生物大分子的可行性。
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