Evaluation of the cell health status is critical for drug screening and cell physiological activity investigations. The existing cell health assessment methods are solely devoted to the study of cell vitality or viability, leading to an incomplete evaluation. Herein, we report a convenient and robust method for the joint assessment of cell viability and vitality based on electric cell-substrate impedance sensing (ECIS) supplied with an environmental temperature control. The static value of electric cell-substrate impedance reflects the survival rate of cells, while the temperature tolerance of cells demonstrates the cell vitality. It was found that the cell vitality evaluated by the temperature tolerance of cells was independent of the initial cell numbers, rendering the proposed method easy to utilize in various applications. We compared the temperature tolerance ECIS method with the traditional trypan blue staining method, the methyl thiazolyl tetrazolium assay, and the direct impedance sensing method for joint evaluation of cell viability and vitality in drug screening. The temperature tolerance ECIS method showed comparable results but with a simpler protocol, faster results, and less dependence on the sample conditions. By providing both information on cell viability and cell vitality, the proposed temperature tolerance ECIS method would pave the way in building a simple and robust sensing system for cell health evaluation.

中文翻译：

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用于联合评估细胞活力和活力的温度耐受电细胞 - 基底阻抗传感

细胞健康状态的评估对于药物筛选和细胞生理活性研究至关重要。现有的细胞健康评估方法仅用于研究细胞活力或活力，导致评估不完整。在此，我们报告了一种方便且稳健的方法，用于基于提供环境温度控制的细胞-基底阻抗传感 (ECIS) 联合评估细胞活力和活力。细胞-底物阻抗的静态值反映了细胞的存活率，而细胞的耐温性则表明了细胞的活力。发现通过细胞的温度耐受性评估的细胞活力与初始细胞数量无关，使得所提出的方法易于在各种应用中使用。我们将耐温 ECIS 方法与传统的台盼蓝染色方法、甲基噻唑基四唑鎓分析和直接阻抗传感方法进行了比较，用于联合评估药物筛选中的细胞活力和活力。温度耐受 ECIS 方法显示了可比较的结果，但协议更简单，结果更快，对样品条件的依赖性更小。通过提供有关细胞活力和细胞活力的信息，所提出的耐温 ECIS 方法将为构建简单而强大的细胞健康评估传感系统铺平道路。温度耐受 ECIS 方法显示了可比较的结果，但协议更简单，结果更快，对样品条件的依赖性更小。通过提供有关细胞活力和细胞活力的信息，所提出的耐温 ECIS 方法将为构建简单而强大的细胞健康评估传感系统铺平道路。温度耐受 ECIS 方法显示了可比较的结果，但协议更简单，结果更快，对样品条件的依赖性更小。通过提供有关细胞活力和细胞活力的信息，所提出的耐温 ECIS 方法将为构建简单而强大的细胞健康评估传感系统铺平道路。