Achondroplasia is a kind of congenital dysplasia due to the defect of endochondral ossification. Achondroplasia is considered to be a protein folding disease leading to endoplasmic reticulum stress. Endoplasmic reticulum stress may lead to disease by affecting the function and survival state of chondrocytes, but the specific mechanism requires further study. In this study, bioinformatics methods, online database mining, screening of differentially expressed genes for pathway enrichment, and interaction analysis were conducted to detect the Wnt family member 5a (Wnt5a) gene. Additionally, we designed a novel DNAzymes-based nanocomposite that can simultaneously silence Wnt5a genes in chondrocytes. The nanocomposite was composed of amino-functionalized cobalt oxyhydroxide nanoflakes modified by DNAzymes that target the Wnt5a gene. Further, we conducted in vitro experiments to verify that Wnt5a can mediate the mitogen-activated protein kinase signaling pathway through the endoplasmic reticulum stress pathway to affect the proliferation of chondrocytes.

中文翻译：

---

使用 DNAzymes-CoOOH 纳米复合材料对 Wnt 家族成员 5a 基因沉默对软骨发育不全增殖的影响。

软骨发育不全是由于软骨内骨化缺陷导致的一种先天性发育不良。软骨发育不全被认为是一种导致内质网应激的蛋白质折叠疾病。内质网应激可能通过影响软骨细胞的功能和生存状态而导致疾病，但具体机制有待进一步研究。本研究采用生物信息学方法、在线数据库挖掘、途径富集的差异表达基因筛选和相互作用分析来检测Wnt家族成员5a（Wnt5a）基因。此外，我们设计了一种新型的基于脱氧核糖核酸酶的纳米复合材料，可以同时沉默软骨细胞中的 Wnt5a 基因。该纳米复合材料由氨基功能化的羟基氧化钴纳米薄片组成，这些薄片由靶向 Wnt5a 基因的 DNA 酶修饰。更远，体外实验验证Wnt5a可以通过内质网应激通路介导丝裂原活化蛋白激酶信号通路影响软骨细胞的增殖。