The potential of flow cytometry for the study of changes in prodigiosin on the cell surface of Serratia marcescens is of academic and practical interest. This is because S. marcescens can produce prodigiosin, a secondary metabolite, with potential use as a cancer-cell inhibitor. In this study, three groups of bacterial cultures with different carbon sources were compared, and the effect of the addition of cAMP to the sucrose-based culture was studied. Both cellular morphology and DNA content were detected by flow cytometry, rendering a broad description of the bacterial behavior. It is the first use of flow cytometry to investigate the dynamics of prodigiosin on the surface of S. marcescens during growth in different media. The fluorescence intensity is related to the DNA content, the forward-scattered light is related to cell volume, and the side-scattered light is related to the surface morphology, especially the surface prodigiosin. These may contribute to the potential development of a bacterial metabolic monitoring strategy using both DNA content analysis and bacterial morphology based on flow cytometry technique.

中文翻译：

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流式细胞仪检测粘质沙雷菌表面灵菌红素的变化

流式细胞术研究粘质沙雷氏菌细胞表面灵菌红素变化的潜力具有学术和实际意义。这是因为S。marcescens可以产生灵菌红素，这是一种次级代谢产物，具有作为癌细胞抑制剂的潜在用途。本研究比较了三组不同碳源的细菌培养物，研究了添加 cAMP 对蔗糖培养物的影响。通过流式细胞术检测细胞形态和 DNA 含量，从而对细菌行为进行广泛描述。这是首次使用流式细胞仪研究灵菌红素在S表面的动力学。粘质在不同媒体的成长过程中。荧光强度与DNA含量有关，前向散射光与细胞体积有关，侧向散射光与表面形态有关，尤其是表面灵菌红素。这些可能有助于利用基于流式细胞术技术的 DNA 含量分析和细菌形态学的细菌代谢监测策略的潜在发展。