Background

Strawberry crinkle virus (SCV) is a member of the genus Cytorhabdovirus, family Rhabdovirida, and order Mononegavirales. SCV affects the production of various strawberry cultivars. In this study we investigated the genetic diversity of SCV in strawberry fields based on P3 (movement protein) gene.

Methods and results

The samples were collected from strawberry fields in the Kurdistan Province, Iran. P3 gene from 20 SCV isolates, representing 18 nucleic acid haplotypes, is composed of 729 nucleotides, encoding a protein with 243 amino acids. SCV-P3 sequences shared 98.77%–99.86% nucleotide and 97.5%–100% amino acid sequence identity. Phylogenetic analyses of the new P3 sequences with two previously published SCV-P3 sequences from the Czech Republic showed that there are two major phylogroups (I and II) and three minor phylogroups in the body of the phylogeny, I-1, I-2, II-1. Comparisons of P3 gene sequences revealed a mutational bias, with more differences being transitions than transversions. The ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that SCV-P3 gene is under predominantly negative selection.

Conclusions

Phylogenetic and sequence identity analyses showed that SCV isolates from Iran are closely related and have not diverged more than 2% based on P3 gene despite geographical separation and strawberry cultivar. This is the first report of the genetic diversity of SCV worldwide.

中文翻译：

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伊朗草莓皱细胞弹状病毒变异使用其运动蛋白 (P3) 基因评估

背景

草莓皱纹病毒(SCV) 是弹状病毒科、弹状病毒科和单倍体病毒目的成员。SCV影响各种草莓品种的生产。在这项研究中，我们基于P3（运动蛋白）基因调查了草莓地中 SCV 的遗传多样性。

方法和结果

这些样品是从伊朗库尔德斯坦省的草莓田中采集的。来自 20 个 SCV 分离株的P3基因，代表 18 个核酸单倍型，由 729 个核苷酸组成，编码具有 243 个氨基酸的蛋白质。SCV-P3 序列具有 98.77%–99.86% 的核苷酸和 97.5%–100% 的氨基酸序列同一性。新的 P3 序列与来自捷克共和国的两个先前发表的 SCV-P3 序列的系统发育分析表明，系统发育主体中有两个主要系统群（I 和 II）和三个次要系统群，I-1、I-2、二-1。P3的比较基因序列揭示了一种突变偏差，其中更多的差异是转变而不是颠换。非同义/同义核苷酸变化的比率<1，表明SCV-P3基因主要处于负选择状态。

结论

系统发育和序列同一性分析表明，尽管地理分离和草莓品种不同，但来自伊朗的 SCV 分离株密切相关，并且基于P3基因的差异不超过 2% 。这是世界范围内首次报道 SCV 的遗传多样性。