Abstract

One of the most informative methods to study the roles of individual proteins in cell functions is based on changing their intracellular concentrations. Genetic knockouts or knockdowns are most commonly used for the purpose. However, acting directly at the level of an expressed protein is more informative or convenient to perform in some cases. This action should ideally be controlled in time and reversible. The review analyzes the current data on systems developed to achieve controlled degradation of proteins via their ubiquitination with subsequent proteasome-mediated degradation or other mechanisms.

中文翻译：

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控制细胞中蛋白质降解的主要方法

摘要

研究单个蛋白质在细胞功能中的作用的最有用的方法之一是基于改变它们的细胞内浓度。基因敲除或敲除最常用于此目的。然而，在某些情况下，直接在表达的蛋白质水平上起作用更能提供信息或方便执行。理想情况下，该动作应及时控制且可逆。该评论分析了有关开发系统的当前数据，该系统通过泛素化以及随后的蛋白酶体介导的降解或其他机制来实现蛋白质的受控降解。