Abstract

The application of array-based oligonucleotides in biological studies is described. These oligonucleotides are mainly used to design large libraries of various nucleotide sequences, which are applied to study protein-nucleic acid interactions, splicing, transcription, translation, and other regulatory processes in mammalian, yeast, and bacterial systems. The application of gene libraries generated by array-based nucleotides along with advanced methods of the combination of DNA duplexes will make it possible to obtain complex genetic designs for synthetic biology.

中文翻译：

---

基于阵列的寡核苷酸在基因设计合成中的应用

摘要

描述了基于阵列的寡核苷酸在生物学研究中的应用。这些寡核苷酸主要用于设计各种核苷酸序列的大型文库，用于研究哺乳动物、酵母和细菌系统中的蛋白质-核酸相互作用、剪接、转录、翻译和其他调控过程。由基于阵列的核苷酸生成的基因文库的应用以及 DNA 双链体组合的先进方法将使合成生物学获得复杂的遗传设计成为可能。