Backgroud

Oxidative stress (OS) can affect the expression of key genes and destroy the intestinal structure. However, it is unclear how OS regulates the expression of circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs.

Objective

The aim of this study was to examine the expression of circRNAs, miRNAs and mRNAs exposed to OS.

Methods

Piglets were exposed to diquat (DQ), a herbicide, and the activity of antioxidant enzymes and the morphology of the intestine were investigated. We utilized whole transcriptome sequencing to examine the global expression of circRNAs, miRNAs and mRNAs in the jejunum.

Results

Compared to controls, 751 circRNAs, 731 miRNAs and 164 mRNAs were differentially expressed in diquat-treated piglets. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that oxidative phosphorylation, RNA degradation and ubiquitin-mediated proteolysis were closely associated with OS.

Conclusions

Our results indicated that diquat-induced OS alters the intestinal structure, resulting in the differential expression of circRNAs, miRNAs and mRNAs in the jejunum of piglets. Meanwhile, OS weakened the enzyme antioxidant system in serum of piglets. Our results provide a foundation for further studies on the mechanisms involved in the response to OS in the jejunum.

中文翻译：

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氧化应激诱导仔猪肠道中circRNAs表达谱的特征

背景

氧化应激（OS）会影响关键基因的表达，破坏肠道结构。然而，尚不清楚 OS 如何调节环状 RNA (circRNA)、microRNA (miRNA) 和 mRNA 的表达。

客观的

本研究的目的是检查暴露于 OS 的 circRNA、miRNA 和 mRNA 的表达。

方法

将仔猪暴露于敌草快 (DQ)，一种除草剂，并研究了抗氧化酶的活性和肠道的形态。我们利用全转录组测序来检查空肠中 circRNA、miRNA 和 mRNA 的整体表达。

结果

与对照组相比，敌草快处理的仔猪有 751 个 circRNA、731 个 miRNA 和 164 个 mRNA 差异表达。京都基因百科全书和基因组通路分析表明，氧化磷酸化、RNA 降解和泛素介导的蛋白水解与 OS 密切相关。

结论

我们的研究结果表明敌草快诱导的 OS 改变了肠道结构，导致仔猪空肠中 circRNA、miRNA 和 mRNA 的差异表达。同时，OS削弱了仔猪血清中的酶抗氧化系统。我们的研究结果为进一步研究空肠对 OS 的反应所涉及的机制提供了基础。