Inflammation is one of the main causes of loss of homeostasis at both the systemic and molecular levels. The aim of this study was to investigate in silico the conservation of inflammation-related proteins in the gilthead seabream (Sparus aurata L.). Open reading frames of the selected genes were used as input in the STRING database for protein–protein interaction network analysis, comparing them with other teleost protein sequences. Proteins of the large yellow croaker (Larimichthys crocea L.) presented the highest percentages of identity with the gilthead seabream protein sequence. The gene expression profile of these proteins was then studied in gilthead seabream specimens subcutaneously injected with carrageenin (1%) or phosphate-buffered saline (control) by analyzing skin samples from the injected zone 12 and 24 h after injection. Gene expression analysis indicated that the mechanisms necessary to terminate the inflammatory response to carrageenin and recover skin homeostasis were activated between 12 and 24 h after injection (at the tested dose). The gene analysis performed in this study could contribute to the identification of the main mechanisms of acute inflammatory response and validate the use of carrageenin as an inflammation model to elucidate these mechanisms in fish.

炎症是系统和分子水平失去稳态的主要原因之一。本研究的目的是在计算机上研究金头鲷 ( Sparus aurata L. ) 中炎症相关蛋白的保存情况。所选基因的开放阅读框被用作 STRING 数据库中蛋白质-蛋白质相互作用网络分析的输入，并将它们与其他硬骨鱼蛋白质序列进行比较。大黄鱼 ( Larimichthys crocea L.) 与金头鲷蛋白质序列的同一性百分比最高。然后通过分析注射后 12 和 24 小时注射区的皮肤样本，在皮下注射角叉菜胶（1%）或磷酸盐缓冲盐水（对照）的金头鲷标本中研究这些蛋白质的基因表达谱。基因表达分析表明，终止对角叉菜胶的炎症反应和恢复皮肤稳态所必需的机制在注射后 12 至 24 小时（在测试剂量下）被激活。本研究中进行的基因分析有助于确定急性炎症反应的主要机制，并验证使用角叉菜胶作为炎症模型来阐明鱼类的这些机制。