The prospection of new degrading enzymes of the plant cell wall has been the subject of many studies and is fundamental for industries, due to the great biotechnological importance of achieving a more efficient depolymerization conversion from plant polysaccharides to fermentable sugars, which are useful not only for biofuel production but also for various bioproducts. Thus, we explored the shotgun metagenome data of a bacterial community (CB10) isolated from sugarcane bagasse and recovered three metagenome-assembled genomes (MAGs). The genomic distance analyses, along with phylogenetic analysis, revealed the presence of a putative novel Chitinophaga species, a Pandoraea nosoerga, and Labrys sp. isolate. The isolation process for each one of these bacterial lineages from the community was carried out in order to relate them with the MAGs. The recovered draft genomes have reasonable completeness (72.67–100%) and contamination (0.26–2.66%) considering the respective marker lineage for Chitinophaga (Bacteroidetes), Pandoraea (Burkholderiales), and Labrys (Rhizobiales). The in-vitro assay detected cellulolytic activity (endoglucanases) only for the isolate Chitinophaga, and its genome analysis revealed 319 CAZymes, of which 115 are classified as plant cell wall degrading enzymes, which can act in fractions of hemicellulose and pectin. Our study highlights the potential of this Chitinophaga isolate provides several plant-polysaccharide-degrading enzymes.

由于实现从植物多糖到可发酵糖的更有效的解聚转化具有重要的生物技术重要性，因此对植物细胞壁的新降解酶的探索已成为许多研究的主题，并且对于工业而言是基础，这不仅可用于生物燃料生产以及各种生物产品。因此，我们探索了从甘蔗渣中分离的细菌群落 (CB10) 的鸟枪宏基因组数据，并恢复了三个宏基因组组装基因组 (MAG)。基因组距离分析以及系统发育分析揭示了一种推定的新型Chitinophaga物种、Pandoraea nosoerga和Labrys 的存在sp. 隔离。对来自群落的这些细菌谱系中的每一种进行分离过程，以便将它们与 MAG 相关联。考虑到Chitinophaga (Bacteroidetes)、Pandoraea (Burkholderiales) 和Labrys ( Rhizobiales) 各自的标记谱系，恢复的基因组草图具有合理的完整性 (72.67–100%) 和污染 (0.26–2.66% )。在体外测定法检测仅在分离纤维素分解活性（内切葡聚糖酶）Chitinophaga，并且它的基因组分析揭示319个CAZymes，其中115被分类为植物细胞壁降解酶，它可以在半纤维素和果胶的级分采取行动。我们的研究强调了这种潜力几丁质分离物提供了几种植物多糖降解酶。