Abstract

Growing antibiotic resistance creates a need to find new antimicrobial agents characterized by diverse chemical structures and pharmaceutical activity. The higher plants synthesize many specialized metabolites as a part of their normal metabolic activity and have been extensively used for centuries in treatment of different diseases. They have a wide activity range depending on the species, topography and climate, and may have different categories of active principles. In contrast to conventional antimicrobial techniques, planar chromatography in combination with biological detection can be an appropriate method of choice for fast, simple, and low-cost screening of plant extract for successful detection of antimicrobial agents which can be good candidates for lead compounds. To date, all bioautography steps such as chromatographic separation, detection with bacteria cells, incubation, and visualization of bioactive bands were improved and optimized. This review gives an overview of bioautography procedure from extraction to structure elucidation of antimicrobial compounds from plants.

摘要

日益增长的抗生素耐药性导致需要寻找具有不同化学结构和药物活性的新型抗菌剂。高等植物合成许多特殊代谢物作为其正常代谢活动的一部分，几个世纪以来一直被广泛用于治疗不同的疾病。根据物种、地形和气候，它们具有广泛的活性范围，并且可能具有不同类别的活性成分。与传统的抗菌技术相比，平面色谱与生物检测相结合可以成为快速、简单和低成本筛选植物提取物以成功检测可作为先导化合物良好候选物的抗菌剂的合适选择方法。迄今为止，所有生物自显影步骤，例如色谱分离，改进和优化了细菌细胞的检测、培养和生物活性条带的可视化。本综述概述了从植物中提取抗菌化合物到结构解析的生物自显影程序。