Periodontal disease disturbs the supportive tissues around the teeth such as connective tissue, gingival tissue, periodontal ligaments and alveolar bone. Previously, treatment of periodontitis was embattled by repopulating the affected site with cells that has capacity to regenerate damaged tissue by endorsing the perception of guided tissue regeneration but it entails additional surgery owing to non-biodegradability. Biodegradable polymeric nanofibrous scaffold imitating extracellular matrix (ECM) delivering functionalized nanoparticles loaded with therapeutic drug have the ability to support cellular functions thereby enhancing regeneration. Present study explores novel amine functionalized zirconia nanoparticle loaded curcumin incorporated SPEEK nanofibrous scaffolds to address periodontal regeneration. Zirconia - crown of dental therapeutics, its amine functionalization further enhanced the strength and cyto-compatibility. Carbon-Silica NMR (59.9 and 69.8 ppm), FT-IR (3426 cm⁻¹), EDAX and XRD (28.9°_, 31.6° and 38.2° pertaining to [−1 1 1], [1 1 1] and [1 2 0] planes) analysis confirmed the effective functionalization of the zirconia nanoparticle with the amine group. Electrospinning was carried out at a voltage of 20 kV and flow rate of 0.05 ml/h. Fabricated nanofibers were highly dense, porous with interconnected fibrous structures that bio-mimic ECM. They exhibited an average diameter of 187 ± 2 nm (SPEEK), 192 ± 2 nm (SPEEK + NH₂–ZrO₂), and 256 ± 17 nm (SPEEK + NH₂–ZrO₂+Cur). Extensively discovered anti-bacterial traits of curcumin supplemented the advantage for the treatment of periodontitis. Incorporated materials improve the physico-chemical, mechanical and biological characteristics of nanofibers. FT-IR, EDAX and XRD analysis of the fabricated nanofibrous scaffold demonstrated the effective incorporation of aminated zirconia loaded curcumin. Results of cyto-compatibility analysis of SPEEK + NH₂–ZrO₂+Cur nanofibrous scaffold depicted a cell viability of 100 ± 1.62%. Results of anti-bacterial assay with zone of inhibition was 6.5 ± 0.5 mm (SPEEK), 7.5 ± 1 mm (SPEEK + NH₂–ZrO₂), and 8 ± 1 mm (SPEEK + NH₂–ZrO₂+Cur). Thus, the fabricated bio-material is cyto-compatible, non-toxic and effective against pathogens exploiting higher potential for periodontal regeneration applications.

牙周病会扰乱牙齿周围的支持组织，例如结缔组织、牙龈组织、牙周韧带和牙槽骨。以前，牙周炎的治疗是通过用具有再生受损组织能力的细胞重新填充受影响的部位来进行的，这些细胞通过认可引导组织再生的感觉，但由于不可生物降解，它需要额外的手术。可生物降解的聚合物纳米纤维支架模拟细胞外基质 (ECM)，提供负载治疗药物的功能化纳米颗粒，具有支持细胞功能从而增强再生的能力。本研究探索了新型胺功能化氧化锆纳米颗粒负载姜黄素纳入 SPEEK 纳米纤维支架以解决牙周再生问题。氧化锆——牙科治疗之冠，其胺功能化进一步增强了强度和细胞相容性。碳-二氧化硅 NMR（59.9 和 69.8 ppm）、FT-IR（3426 cm ^-1）、EDAX 和 XRD（与 [-1 1 1]、[1 1 1] 和 [1] 相关的28.9° _、 31.6° 和 38.2° 2 0] 平面）分析证实了氧化锆纳米颗粒与胺基团的有效功能化。静电纺丝在20kV的电压和0.05ml/h的流速下进行。制造的纳米纤维是高度致密的、多孔的，具有可仿生 ECM 的互连纤维结构。它们的平均直径为 187 ± 2 nm (SPEEK)、192 ± 2 nm (SPEEK + NH ₂ –ZrO ₂) 和 256 ± 17 nm (SPEEK + NH ₂ –ZrO ₂ + Cur )。广泛发现的姜黄素抗菌特性补充了治疗牙周炎的优势。掺入的材料改善了纳米纤维的物理化学、机械和生物特性。制备的纳米纤维支架的 FT-IR、EDAX 和 XRD 分析证明了胺化氧化锆负载姜黄素的有效掺入。SPEEK + NH ₂ –ZrO ₂ + Cur的细胞相容性分析结果纳米纤维支架描绘了 100 ± 1.62% 的细胞活力。具有抑菌圈的抗菌试验结果为 6.5 ± 0.5 mm (SPEEK)、7.5 ± 1 mm (SPEEK + NH ₂ –ZrO ₂ ) 和 8 ± 1 mm (SPEEK + NH ₂ –ZrO ₂ + Cur )。因此，所制造的生物材料具有细胞相容性、无毒和有效对抗病原体的特性，在牙周再生应用中具有更高的潜力。