The European lobster (Homarus gammarus) forms the base of an important fishery along the coasts of Europe. However, stocks have been in decline for many years, prompting new regulations in the fishery and also restocking efforts. An important feature of any restocking effort is the assessment of success in the number of released juveniles that stay and become adult over time. Here, we tested the power of a single nucleotide polymorphism (SNP) DNA marker panel developed for population assignment to correctly infer parentage on the maternal side of lobster larvae, in the absence of known fathers, using lobsters included in a current restocking effort on the Swedish west coast. We also examined the power to reconstruct the unknown paternal genotypes, and examined the number of fathers for each larval clutch. We found that the 96-SNP panel, despite only containing 78 informative markers, allowed us to assign all larvae to the correct mother. Furthermore, with ten genotyped larvae or more, confident paternal genotypes could be reconstructed. We also found that 15 out of 17 clutches were full siblings, whereas two clutches had two fathers. To our knowledge, this is the first time a SNP panel of this size has been used to assess parentage in a crustacean restocking effort. Our conclusion is that the panel works well, and that it could be an important tool for the assessment of restocking success of H. gammarus in the future.

欧洲龙虾 ( Homarus gammarus) 形成了欧洲沿海重要渔业的基地。然而，多年来库存一直在下降，促使渔业制定新规定并进行重新放养。任何重新放养努力的一个重要特征是评估释放的幼鱼数量随着时间的推移留下并成为成年的成功率。在这里，我们测试了为种群分配而开发的单核苷酸多态性 (SNP) DNA 标记面板的功效，以在没有已知父亲的情况下正确推断龙虾幼虫母体的亲子关系，使用当前重新放养工作中的龙虾瑞典西海岸。我们还检查了重建未知父本基因型的能力，并检查了每个幼虫窝的父亲数量。我们发现 96-SNP panel 尽管只包含 78 个信息标记，允许我们将所有幼虫分配给正确的母亲。此外，用十个或更多基因分型的幼虫，可以重建可靠的父本基因型。我们还发现，17 个孩子中有 15 个是兄弟姐妹，而两个孩子有两个父亲。据我们所知，这是第一次使用这种规模的 SNP 面板来评估甲壳类动物放养工作中的亲子关系。我们的结论是该小组运作良好，并且它可能是评估重新放养成功的重要工具 这是第一次使用这种规模的 SNP 面板来评估甲壳类动物放养工作中的亲子关系。我们的结论是该小组运作良好，并且它可能是评估重新放养成功的重要工具 这是第一次使用这种规模的 SNP 面板来评估甲壳类动物放养工作中的亲子关系。我们的结论是该小组运作良好，并且它可能是评估重新放养成功的重要工具H . 伽玛鲁斯在未来。