Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Complex,Biochemistry

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Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Complex
Biochemistry ( IF 2.9 ) Pub Date : 2021-08-27 , DOI: 10.1021/acs.biochem.1c00462
Kenji V P Nagashima ₁ , Sakiko Nagashima ₁ , Masaharu Kitashima ₂ , Kazuhito Inoue _{1,

2} , Michael T Madigan ₃ , Yukihiro Kimura ₄ , Zheng-Yu Wang-Otomo ₅

Affiliation

Light-harvesting complex 1 (LH1) of the thermophilic purple sulfur bacterium Thermochromatium tepidum can be expressed in the purple non-sulfur bacterium Rhodobacter sphaeroides and forms a functional RC-LH1 complex with the native Rba. sphaeroides reaction center (Nagashima, K. V. P., et al. Proc. Natl. Acad. Sci. U. S. A. 2017, 114, 10906−10911). Although there is a large uphill energy gap between Tch. tepidum LH1 and the Rba. sphaeroides RC in this chimeric complex, it has been shown that light energy can be transferred, consistent with that seen in the native Rba. sphaeroides RC-LH1 complex. In this study, the contribution of this chimeric complex to growth and photosynthetic energy conversion in the hybrid organism was quantified. The mutant synthesizing this chimeric complex was grown phototrophically under 940 nm light-emitting diode (LED) light preferentially absorbed by Tch. tepidum LH1 and showed faster growth at low intensities of this wavelength than both a mutant strain of Rba. sphaeroides lacking LH2 and a mutant lacking all light-harvesting complexes. When grown with 850 nm LED light, the strain containing the native Rba. sphaeroides LH1-RC grew faster than the chimeric strain. Electron transfer from the RC to the membrane-integrated cytochrome bc₁ complex was also estimated by flash-induced absorption changes in heme b. The rate of ubiquinone transport through the LH1 ring structure in the chimeric strain was virtually the same as that in native Rba. sphaeroides. We conclude that Tch. tepidum LH1 can perform the physiological functions of native LH1 in Rba. sphaeroides.

中文翻译：

含有 Thermochromatium tepidum 光捕获复合物 1 和球形红杆菌反应中心复合物的工程光养生物的光合生长和能量转换

嗜热紫色硫细菌Thermochromatium tepidum的捕光复合物 1 (LH1)可在紫色非硫细菌球形红细菌中表达，并与天然Rba形成功能性 RC-LH1 复合物。球状反应中心（长岛，KVP，等。过程国家队阿卡德。科学。美国 2017年，114，10906-10911）。尽管Tch之间存在较大的上坡能隙。tepidum LH1 和Rba。sphaeroides RC 在这个嵌合复合物中，已经表明光能可以转移，与在天然Rba中看到的一致。sphaeroides RC-LH1 复合体。在这项研究中，量化了这种嵌合复合物对杂交生物体生长和光合能量转换的贡献。合成这种嵌合复合物的突变体在 940 nm 发光二极管 (LED) 光下光养生长，该光优先被Tch吸收。温热的LH1 和在该波长的低强度下显示出比Rba突变株更快的生长。缺乏 LH2 的sphaeroides和缺乏所有捕光复合物的突变体。当在 850 nm LED 灯下生长时，菌株含有天然Rba。sphaeroides LH1-RC 比嵌合菌株生长得更快。从 RC 到膜整合的细胞色素bc ₁复合物的电子转移也通过血红素b 中闪光诱导的吸收变化来估计。在嵌合菌株中通过 LH1 环结构运输泛醌的速率实际上与天然Rba 中的相同。球状体。我们得出结论，Tch。温热的LH1 可以在Rba 中执行天然 LH1 的生理功能。球状体。

更新日期：2021-09-14

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