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Phyto-fabrication, characterization, and biomedical activity of silver nanoparticles mediated from an epiphytic plant Luisia tenuifolia Blume
Applied Nanoscience Pub Date : 2021-08-27 , DOI: 10.1007/s13204-021-02022-z
Mathiyazhagan Narayanan 1 , M. Deepika 2 , Devarajan Natarajan 2 , Ying Ma 3 , Omaima Nasif 4 , Sulaiman Ali Alharbi 5 , Ramalingam Srinivasan 6
Affiliation  

The purpose of this study was to assess the ability of an aqueous leaf extract of Luisia tenuifolia to synthesize silver nanoparticles (AgNPs) and their antimicrobial and Aedes aegypti larvicidal activity. The presence of visible color changes ranging from pale yellow to dark brownish confirmed the presence of AgNPs in the reaction mixture. A sharp absorbance peak at 420 nm was found using a UV–visible spectrophotometer, confirming the presence of AgNPs. X-ray Diffraction (XRD) and Fourier-Transform Infrared Spectroscopy (FTIR) analyses were used to investigate the crystalline structure and active functional groups involved in the reduction of AgNPs. The synthesized AgNPs were examined using High Resolution Transmission Electron Microscopy (HR-TEM), and it was discovered that the AgNPs were spherical in shape, with sizes ranging from 10 to 30 nm and a diameter of 0.241 nm. The crystalline structure of silver particles (face-centered cubic (fcc)) was confirmed by Selected Area Electron Diffraction (SAED) pattern analysis, which revealed concentric rings and blinking dots. At the highest concentrations (150 µg mL−1), the synthesized AgNPs have significant antimicrobial potential against some common bacterial pathogens such as Salmonella paratyphi (31 mm), Staphylococcus aureus (28 mm), Escherichia coli (20 mm), and Bacillus subtilis (23 mm), as well as phytopathogenic fungi such as Colletotrichum falcatum (12 mm), Fusarium sp. (12 mm), Aspergillus oryzae (10 mm), and Trichoderma sp. (10 mm) at the highest concentration (150 µg mL−1) of AgNPs. Fortunately, the synthesized AgNPs also have significant larvicidal potential against 1st and 2nd instar larvae of Ae. aegypti. Thus, aqueous leaf extract of Luisia tenuifolia synthesized AgNPs can be used to benefit humanity.



中文翻译:

由附生植物 Luisia tenuifolia Blume 介导的银纳米粒子的植物制造、表征和生物医学活性

本研究的目的是评估细叶 Luisia的水叶提取物合成银纳米粒子 (AgNPs) 及其抗菌剂和埃及伊蚊的能力。杀幼虫活性。从淡黄色到深褐色的可见颜色变化的存在证实了反应混合物中存在 AgNPs。使用紫外可见分光光度计在 420 nm 处发现了一个尖锐的吸收峰,证实了 AgNPs 的存在。X 射线衍射 (XRD) 和傅里叶变换红外光谱 (FTIR) 分析用于研究参与 AgNPs 还原的晶体结构和活性官能团。使用高分辨率透射电子显微镜 (HR-TEM) 检查合成的 AgNPs,发现 AgNPs 呈球形,尺寸范围为 10 至 30 nm,直径为 0.241 nm。银颗粒(面心立方(fcc))的晶体结构通过选区电子衍射(SAED)图案分析得到证实,这揭示了同心环和闪烁的点。在最高浓度 (150 µg mL-1 ),合成的 AgNPs 对一些常见的细菌病原体具有显着的抗菌潜力,例如副伤寒沙门氏菌(31 mm)、金黄色葡萄球菌(28 mm) 、大肠杆菌(20 mm) 和枯草芽孢杆菌(23 mm),以及植物病原真菌,如Colletotrichum falcatum (12 mm)、Fusarium sp。(12 毫米)、米曲霉(10 毫米)和木霉属。(10 mm) 在最高浓度 (150 µg mL -1 ) 的 AgNPs 下。幸运的是,合成的AgNPs也有显著杀虫潜力反对1和2Ae 的幼虫埃及人。因此,细叶 Luisia tenuifolia 的水叶提取物合成的 AgNPs 可用于造福人类。

更新日期:2021-08-27
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