The synthesis, characterization and catalytic activities of two homodinuclear Cu(II) and Zn(II) complexes of a carboxylate-rich ligand, N,N′-Bis[2-carboxybenzomethyl]-N,N′ -Bis[carboxymethyl]-1,3-diaminopropan-2-ol (H₅ccdp) ligand towards the hydrolysis of (p-nitrophenyl phosphate) (PNPP) and bis(p-nitrophenyl) phosphate (BNPP) substrates in aqueous systems are described. Kinetic investigations were carried out using UV–Vis spectrophotometric techniques at 25 °C and 37 °C and different pH (7–10) conditions. The kinetic studies revealed that the turnover rate (k_cat) values among the PNPP hydrolysis systems, the highest and the lowest k_cat values were displayed by [Cu₂(ccdp)(μ-OAc)]²⁻ at 2.34 × 10⁻⁶ s⁻¹ (pH 8 and 37 °C) and 2.13 × 10⁻⁸ s⁻¹ (pH 8 and 25 °C), respectively. However, similar comparisons among the BNPP hydrolysis revealed that highest and the lowest k_cat values were displayed by [Zn₂(ccdp)(μ-OAc)]²⁻ at 4.64 × 10⁻⁸ s⁻¹ (pH 9 and 37 °C) and 2.38 × 10⁻⁹ (pH 9 and 25 °C). Significantly enough, the catalyst−substrate adduct species containing a metal bound PNPP and BNPP have been detected by ESI-MS techniques. Additionally, a PNPP-bound copper complex has been isolated and crystalized using single crystal X-ray diffraction technique. Based on the structural and activity information obtained in this study, reaction mechanisms for the hydrolysis of PNPP have been proposed.

富含羧酸盐的配体N,N' -Bis[2-carboxybenzomethyl]- N,N' -Bis[carboxymethyl]-1的两种同双核 Cu(II) 和 Zn(II) 配合物的合成、表征和催化活性描述了 ,3-diaminopropan-2-ol (H ₅ ccdp) 配体在水性体系中对 ( p -nitrophenyl phosphate) (PNPP) 和双( p - nitrophenyl) phosphate (BNPP) 底物的水解。在 25 °C 和 37 °C 和不同 pH (7-10) 条件下，使用 UV-Vis 分光光度计技术进行动力学研究。动力学研究表明，在 PNPP 水解系统中，周转率 ( k _cat ) 值最高和最低k _cat值由 [Cu ₂ (ccdp)( μ -OAc)] ^2-在 2.34 × 10 ^-6  s ^-1 (pH 8 和 37 °C) 和 2.13 × 10 ^-8  s ^-1 (pH 8 和25 °C），分别。然而，BNPP 水解之间的类似比较表明，在 4.64 × 10 ^-8  s ^-1（pH 9 和 37 °C ）下，[Zn ₂ (ccdp)( μ -OAc)] ^{2-显示出最高和最低的}k _cat值) 和 2.38 × 10 ^-9（pH 9 和 25 °C）。值得注意的是，ESI-MS 技术已检测到含有金属结合的 PNPP 和 BNPP 的催化剂-底物加合物物质。此外，已使用单晶 X 射线衍射技术分离并结晶了 PNPP 结合的铜络合物。根据本研究中获得的结构和活性信息，提出了 PNPP 水解的反应机理。