Gastric cancer cell-derived exosomes as biomarkers have a very high application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30 ∼ 150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). Here we proposed a highly integrated exosome separation and detection (ExoSD) chip to immunomagnetic separate exosomes from cell culture supernatant in a manner of continuous flow, and to immunofluorescence detect gastric cancer cell-derived exosomes with high sensitivity. The ExoSD chip has achieved a high exosome recovery (>80%) and purity (>83%) at the injection rate of 4.8 mL/h. Furthermore, experimental results based on clinical serum samples of patients with gastric cancer (stages I and II) show that the detection rate of the ExoSD chip is as high as 70 %. The proposed ExoSD chip has been successfully demonstrated as a cutting-edge platform for exosomes separation and detection. It can be served as a versatile platform to extend to the applications of separation and detection of the other cell-derived exosomes or cells.

胃癌细胞来源的外泌体作为生物标志物在早期胃癌的无创检测中具有非常高的应用潜力。然而，外泌体的小尺寸（30~150 nm）导致将它们从复杂介质（例如血浆、尿液、唾液和细胞培养上清液）中分离和检测的巨大挑战。在这里，我们提出了一种高度集成的外泌体分离和检测（ExoSD）芯片，以连续流动的方式从细胞培养上清液中免疫磁性分离外泌体，并以高灵敏度免疫荧光检测胃癌细胞来源的外泌体。ExoSD 芯片在 4.8 mL/h 的注射速率下实现了高外泌体回收率 (>80%) 和纯度 (>83%)。此外，基于胃癌患者（I期和II期）临床血清样本的实验结果表明，ExoSD芯片的检出率高达70%。所提出的 ExoSD 芯片已成功证明是外泌体分离和检测的前沿平台。它可以作为一个多功能平台，扩展到其他细胞来源的外泌体或细胞的分离和检测应用。