Anu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma. It contains more than 25 herbs and goat milk as per the Ayurvedic Formulary of India (AFI). It strengthens the neck, shoulder, and chest muscles and improves the capacity of sense organs. It delays the aging process and reduces hair fall. Recent studies showed that it is also useful in COVID-19. In the current study, an attempt to develop quality control protocols and evaluate the standardization parameters like refractive index, iodine value, saponification value, peroxide value, acid value, rancidity, HPTLC fingerprint profile along with major bioactive compound and quantification of Berberine chloride, Negundoside, and Marmelosin by HPLC. Establishing quality protocol and standard parameters like physicochemical parameters and estimation of bioactive compounds of this preparation is significant for quality control. In this study, HPTLC identifies bioactive chemical compounds like Berberine chloride, Marmelosin, Negundoside, glycyrrhizin, and para hydroxybenzoic acid (PHBA), Lupeol, Embelin, and Solasodine, which were present in the Anu Taila formulation. HPLC was used to estimate the bioactive marker compounds Negundoside, Berberine chloride, and Marmelosin were present in the Anu Taila formulation. The quantitative evaluation of Berberine chloride (0.0013%), Marmelosin (0.0366%), Negundoside (0.0086%) is present in Anu Taila formulation. The study reveals that sufficient quality control parameters were followed during the preparation of the formulation. Physicochemical analysis was carried out as per the guidelines of Ayurvedic Pharmacopeia of India. HPTLC and HPLC profiles generated in this particular study can be considered as a preliminary tool ascertaining the authenticity of Anu Taila.

中文翻译：

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通过 HPLC 和 HPTLC 鉴定和估计生物活性成分 Negundoside、氯化小檗碱和 Marmelosin，以制定阿育吠陀药油制剂的质量控制方案

Anu Taila 是一种古老的药油 Ayurvedic 制剂，通常用于 nasya 业力。根据印度阿育吠陀处方集 (AFI)，它含有超过 25 种草药和山羊奶。它可以加强颈部、肩部和胸部肌肉，提高感觉器官的能力。它可以延缓衰老过程并减少头发掉落。最近的研究表明，它在 COVID-19 中也很有用。在目前的研究中，试图开发质量控制协议并评估标准化参数，如折射率、碘值、皂化值、过氧化值、酸值、酸败、HPTLC 指纹图谱以及主要生物活性化合物和氯化小檗碱、Negundoside 的量化, 和 Marmelosin 通过 HPLC。建立质量方案和标准参数，如该制剂的物理化学参数和生物活性化合物的估计，对于质量控制具有重要意义。在这项研究中，HPTLC 鉴定了 Anu Taila 制剂中存在的生物活性化合物，如氯化小檗碱、Marmelosin、Negundoside、甘草甜素和对羟基苯甲酸 (PHBA)、羽扇豆醇、Embelin 和 Solaso​​dine。HPLC 用于评估 Anu Taila 制剂中存在的生物活性标记化合物 Negundoside、氯化小檗碱和 Marmelosin。Anu Taila 制剂中存在氯化小檗碱 (0.0013%)、Marmelosin (0.0366%)、Negundoside (0.0086%) 的定量评估。研究表明，在制剂制备过程中遵循了足够的质量控制参数。理化分析按照印度阿育吠陀药典指南进行。在这项特定研究中生成的 HPTLC 和 HPLC 配置文件可被视为确定 Anu Taila 真实性的初步工具。