The ePlex® and QIAstat-Dx® respiratory pathogen panels detect multiple respiratory pathogens, mainly viruses but also Legionella pneumophila, Mycoplasma pneumoniae and Bordetella pertussis. The assays have been marketed for use in nasopharyngeal swab specimens. For diagnosing bacterial pneumonia, lower respiratory tract (LRT) specimens are indicated. Aim of this study was to evaluate the performance of these syndromic panels for these three bacterial targets in samples from the LRT. Fifty-six specimens were collected from our repositories, five negative samples and fifty-one samples which had been previously tested positive with the routine diagnostic real-time PCR assays for Legionella spp. (N = 20), Bordetella spp. (N = 16) or M. pneumoniae (N = 15). The QIAstat-Dx Respiratory Panel V2 (RP) assay detected all of the L. pneumophila and B. pertussis positive samples but only 11/15 (73.3 %) of the M. pneumoniae targets. The ePlex Respiratory Pathogen Panel (RPP) assay detected 10/14 (71.4 %) of the L. pneumophila targets, 8/12 (66.7 %) of the B. pertussis positive samples and 13/15 (86.7 %) of the M. pneumoniae targets. No false-positive results were reported for all three bacterial pathogens by both assays. The clinical performance of both assays depended highly on the bacterial load in the sample and the type of specimen under investigation.

中文翻译：

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评估 Genmark ePlex® 和 QIAstat-Dx® 呼吸道病原体检测下呼吸道标本中的细菌靶标

ePlex® 和 QIAstat-Dx® 呼吸道病原体面板可检测多种呼吸道病原体，主要是病毒，但也包括嗜肺军团菌、肺炎支原体和百日咳博德特氏菌。该测定已上市用于鼻咽拭子样本。为了诊断细菌性肺炎，需要下呼吸道 (LRT) 标本。本研究的目的是评估这些综合征组对 LRT 样品中这三种细菌靶标的性能。从我们的储存库中收集了 56 个样本，5 个阴性样本和 51 个先前用军团菌属的常规诊断实时 PCR 检测检测为阳性的样本。（N = 20），博代氏菌属。(N = 16) 或肺炎支原体 (N = 15)。QIAstat-Dx Respiratory Panel V2 (RP) 检测检测到所有嗜肺军团菌和 B. 百日咳阳性样本，但只有 11/15 (73.3 %) 的肺炎支原体目标。ePlex Respiratory Pathogen Panel (RPP) 检测检测到 10/14 (71.4 %) 的嗜肺军团菌靶标、8/12 (66.7 %) 的百日咳杆菌阳性样本和 13/15 (86.7 %) 的 M. pneumophila 靶标。肺炎靶点。两种测定均未报告所有三种细菌病原体的假阳性结果。两种检测的临床表现在很大程度上取决于样本中的细菌载量和所研究样本的类型。两种测定均未报告所有三种细菌病原体的假阳性结果。两种检测的临床表现在很大程度上取决于样本中的细菌载量和所研究样本的类型。两种测定均未报告所有三种细菌病原体的假阳性结果。两种检测的临床表现在很大程度上取决于样本中的细菌载量和所研究样本的类型。