Single-beam dual-color alternate-pathway two-photon spectroscopy: Toward an optical toolbox for redox biology,Journal of Raman Spectroscopy

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Single-beam dual-color alternate-pathway two-photon spectroscopy: Toward an optical toolbox for redox biology
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2021-08-26 , DOI: 10.1002/jrs.6183
Artem S. Chebotarev ₁ , Aleksandr A. Lanin _{1,

2,

3} , Roman I. Raevskii _{4,

5} , Alexander I. Kostyuk _{5,

6} , Daria D. Smolyarova _{4,

5} , Dmitry S. Bilan _{5,

6} , Ilya V. Savitskii ₁ , Andrei B. Fedotov _{1,

2,

3,

7} , Vsevolod V. Belousov _{5,

6,

8} , Aleksei M. Zheltikov _{1,

2,

3,

9}

Affiliation

We demonstrate a versatile single-laser platform for single-beam dual-color two-photon spectroscopy that combines a short-pulse laser source with a tunable broadband wavelength converter based on a highly nonlinear photonic-crystal fiber (PCF). We show that the short-pulse PCF output can be tailored, via dispersion and nonlinearity management, to deliver a broadband optical driver whose spectral structure is ideally suited for a single-beam two-photon absorption (2PA) spectroscopy of the next-generation genetically encodable fluorescent-protein (FP) sensors of pH and redox-responsive contrast agents. As a promising spectroscopic resource for redox biology, the short-pulse PCF output can be finely sculpted to alternately drive an FP system via a one of its two 2PA-allowed quantum pathways, yielding a high-contrast fluorescence readout for a highly sensitive detection of redox reactions and signaling, pH sensing, and oxidative-stress diagnosis.

中文翻译：

单光束双色交替通路双光子光谱：迈向氧化还原生物学的光学工具箱

我们展示了用于单光束双色双光子光谱的多功能单激光器平台，该平台将短脉冲激光源与基于高度非线性光子晶体光纤 (PCF) 的可调谐宽带波长转换器相结合。我们表明，短脉冲 PCF 输出可以通过色散和非线性管理进行定制，以提供宽带光学驱动器，其光谱结构非常适合下一代遗传学的单光束双光子吸收 (2PA) 光谱。 pH 值和氧化还原响应造影剂的可编码荧光蛋白 (FP) 传感器。作为氧化还原生物学的一种有前途的光谱资源，可以精细雕刻短脉冲 PCF 输出，以通过其两个 2PA 允许的量子途径之一交替驱动 FP 系统，

更新日期：2021-09-13

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