The purpose of this study was to evaluate the effects of β-conglycinin on intestinal permeability, intestinal morphology and the expression of tight junction protein in Rhynchocypris lagowski Dybowski. 750 Rhynchocypris lagowski Dybowski (4.61 ± 0.01 g) were divided into 5 groups (CK, β-20, β-40, β-60 and β-80 groups) and fed with 5 diets contained, respectively (0, 20, 40, 60 and 80 g/kg), β-conglycinin for 8 weeks. These results showed dietary β-conglycinin could destroy the structural integrity of intestine, cause villus to break and shrink, and the epithelium and lamina propria to separate. Simultaneously, compared with CK group, the levels of Diamine oxidase (DAO), 5-Hydroxytryptamine (5-HT), D-lactic acid (D-LA) and Endothelin-1 (ET-1) in serum were significantly increased in β-20, β-40, β-60 and β-80 groups (P < .05). In proximal intestines (PI), the expression of Zonula occludens-1 (ZO-1) and Occludin-b was significantly reduced, and lamina propria width was significantly increased in β-60 and β-80 groups (P < .05), and fold height, muscular layer thickness and the expression of Claudin were significantly decreased in β-40, β-60 and β-80 groups (P < .05), and the expression of Occludin-a was significantly decreased in β-20, β-40, β-60 and β-80 groups (P < .05); in addition, the expression of ZO-1 in PI was significantly increased in β-20 and β-40 groups (P < .05). In mid intestines (MI), fold height and the expression of ZO-1 and Claudin were significantly reduced, and lamina propria width was significantly increased in β-40, β-60 and β-80 groups (P < .05), and the expression of ZO-1 was significantly increased in β-20 groups (P < .05), and muscular layer thickness and the expression of Occludin-a and Occludin-b were significantly decreased in β-20, β-40, β-60 and β-80 groups (P < .05). In distal intestines (DI), fold height and the expression of ZO-1, Claudin, Occludin-a and Occludin-b were significantly reduced, and lamina propria width was significantly increased in β-20, β-40, β-60 and β-80 groups (P < .05), and muscular layer thickness was significantly reduced in β-40, β-60 and β-80 groups (P < .05). In summary, dietary β-conglycinin had negative effects on tight junctions, intestinal permeability and morphology. Moreover, the negative effects of β-conglycinin on intestines from high to low were DI, MI and PI in our study.

中文翻译：

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β-伴大豆球蛋白对Rhynchocypris lagowski Dybowski肠道结构和肠道通透性的影响

本研究旨在评估β-伴大豆球蛋白对Rhynchocypris lagowski Dybowski肠道通透性、肠道形态和紧密连接蛋白表达的影响。第750章Dybowski (4.61 ± 0.01 g) 分为 5 组（CK、β-20、β-40、β-60 和 β-80 组），分别饲喂含有 5 种日粮（0、20、40、60 和 80 g/kg), β-伴大豆球蛋白 8 周。这些结果表明，膳食β-伴大豆球蛋白会破坏肠道的结构完整性，导致绒毛破裂和收缩，上皮和固有层分离。同时，与 CK 组相比，β -20、β-40、β-60 和 β-80 组 ( P  < .05)。在近端肠（PI）中，β-60和β-80组Zonula occludens-1（ZO-1）和Occludin-b的表达明显降低，固有层宽度明显增加（P < .05)，β-40、β-60和β-80组褶皱高度、肌层厚度和Claudin表达显着降低( P  < .05)，Occludin-a表达显着降低在 β-20、β-40、β-60 和 β-80 组中 ( P  < .05)；此外，β-20和β-40组PI中ZO-1的表达显着增加（P  < .05）。在中肠（MI），β-40、β-60和β-80组褶皱高度和ZO-1和Claudin的表达显着降低，固有层宽度显着增加（P  < .05）， β-20 组 ZO-1 表达显着升高（P < .05)，β-20、β-40、β-60和β-80组肌层厚度和Occludin-a、Occludin-b表达明显降低( P  < .05)。在远端肠（DI）中，折叠高度和 ZO-1、Claudin、Occludin-a 和 Occludin-b 的表达显着降低，β-20、β-40、β-60 和β-80组（P  < .05），β-40、β-60和β-80组肌层厚度显着降低（P  < .05）。总之，膳食β-伴大豆球蛋白对紧密连接、肠道通透性和形态有负面影响。此外，在我们的研究中，β-伴大豆球蛋白对肠道的负面影响从高到低依次为DI、MI和PI。