The development of new bone adhesives is severely restricted due to the lack of a standard test method to examine their bond strength. Therefore, this study presents an in vitro bone-to-bone adhesion test method based on a compression shear test using cortical bone specimens (35 × 15 × 3 mm). The zero-gap lap joint test configuration was used to measure the bonding strengths of two soft tissue adhesives (Histoacryl® and BSA-Glue) and a bone cement (Palacos® LV). For reproducible bonding results, it is crucial to work with specimen having reproducible surface, dimensions and where and how they were taken from a bone. Therefore, this is a focus of the work. The adhesive strengths were determined under dry and physiological conditions to evaluate the reliability of the test procedure. Furthermore, the influence of previous acid etching of the bone surface was also investigated.

The adhesives achieved bond strengths of 5.5 MPa (Histoacryl®), 3.2 MPa (BSA-Glue) and 2.8 MPa (Palacos® LV) under dry conditions. Under physiological conditions, the measured adhesive strengths of all three bonding materials decreased by around 90%. The acid etching led to a higher presence of reactive amino groups on the bone surface and improved the adhesion of Histoacryl® and BSA-Glue. The Palacos® LV bone cement suffered a reduction in adhesion as the acid smoothened the bone surface, resulting in less mechanical interlocking.

The proposed test method is based on known standards and enables the usability of medical adhesives as a bone adhesive to be evaluated in a reproducible manner.

由于缺乏检查其粘合强度的标准测试方法，新型骨粘合剂的开发受到严重限制。因此，本研究提出了一种体外基于使用皮质骨标本 (35 × 15 × 3 mm) 进行压缩剪切试验的骨间粘附试验方法。零间隙搭接接头测试配置用于测量两种软组织粘合剂（Histoacryl® 和 BSA-Glue）和一种骨水泥（Palacos® LV）的粘合强度。为了获得可重现的粘合结果，使用具有可重现表面、尺寸以及它们从骨骼中取出的位置和方式的样本至关重要。因此，这是工作的重点。在干燥和生理条件下测定粘合强度以评估测试程序的可靠性。此外，还研究了先前酸蚀对骨表面的影响。

粘合剂在干燥条件下达到 5.5 MPa (Histoacryl®)、3.2 MPa (BSA-Glue) 和 2.8 MPa (Palacos® LV) 的粘合强度。在生理条件下，所有三种粘合材料的测量粘合强度下降了约 90%。酸蚀导致骨表面活性氨基的含量更高，并提高了 Histoacryl® 和 BSA-Glue 的附着力。Palacos® LV 骨水泥的粘附力降低，因为酸使骨表面光滑，导致机械互锁减少。

建议的测试方法基于已知标准，并且能够以可重复的方式评估医用粘合剂作为骨粘合剂的可用性。