Currently, it is clear that the luxS gene has an impact on the process of biofilm formation in Campylobacter jejuni. However, even within the species, naturally occurring strains of Campylobacter lacking the luxS gene exist, which can form biofilms. In order to better understand the genetic determinants and the role of quorum sensing through the LuxS/AI-2 pathway in biofilm formation, a set of mutant/complemented strains of C. jejuni 81–176 were prepared. Additionally, the impact of the mutagenic strategy used against the luxS gene was investigated. Biofilm formation was affected by both the presence and absence of the luxS gene, and by the mutagenic strategy used. Analysis by CLSM showed that all mutant strains formed significantly less biofilm mass when compared to the wild-type. Interestingly, the deletion mutant (∆luxS) showed a larger decrease in biofilm mass than the substitution (∙luxS) and insertional inactivated (\(\colon\colon\)luxS) mutants, even though all the mutant strains lost the ability to produce autoinducer-2 molecules. Moreover, the biofilm of the ∆luxS mutant lacked the characteristic microcolonies observed in all other strains. The complementation of all mutant strains resulted in restored ability to produce AI-2, to form a complex biofilm, and to develop microcolonies at the level of the wild-type.

目前，很明显luxS基因对空肠弯曲杆菌的生物膜形成过程有影响。然而，即使在物种内部，也存在缺乏luxS基因的自然产生的弯曲杆菌菌株，它们可以形成生物膜。为了更好地了解遗传决定因素和通过 LuxS/AI-2 途径的群体感应在生物膜形成中的作用，制备了一组空肠弯曲菌 81-176 的突变/互补菌株。此外，还研究了针对luxS基因使用的诱变策略的影响。生物膜的形成受到luxS存在和不存在的影响基因，并通过使用的诱变策略。CLSM 分析表明，与野生型相比，所有突变菌株形成的生物膜质量显着减少。有趣的是，缺失突变体 (Δ luxS ) 显示出比替换 (∙ luxS ) 和插入失活 ( \(\colon\colon\) luxS ) 突变体更大的生物膜质量减少，即使所有突变菌株都失去了生产能力自诱导剂 2 分子。此外，ΔluxS突变体的生物膜缺乏在所有其他菌株中观察到的特征性微菌落。所有突变菌株的互补导致恢复产生 AI-2、形成复杂生物膜和在野生型水平上形成微菌落的能力。