While liquid biopreparations for water and soil bioremediation are convenient and economically attractive, microbial survival under standard environmental conditions is poor. Microbial immobilization is a common and efficient method to preserve high viable cell titers. We tested the effect of three new materials on the survival of various hydrocarbon oxidizers during long-term storage. The suspended (planktonic) cultures stored under the same conditions served as a control when assessing the viability of immobilized cultures after long-term storage. The cells of Acinetobacter seifertii, Pseudomonas extremaustralis, P. aeruginosa, Rhodococcus erythropolis, and Dietzia maris grown with microcapsules (MCs) of chitosan-modified polyurea (represented by crumpled spheres, 40–200 µm in diameter) attached abundantly to the MC surface. After several months of storage with MCs, CFU titers were two to five times higher than in the control. Cultivation of P. aeruginosa and R. erythropolis with polylactide (PLA) MCs (represented by slightly crumpled thin-walled spheres 25–100 µm in size) resulted in the formation of thick biofilms on MC surface. After 30-day storage with these MCs, CFU titers of R. erytropolis were up to one thousand times higher than in the control. In the presence of PLA MCs, the respiration rates of both cultures were five to eight times higher than in the control without MCs. The reasons of the better survival of immobilized cells are being discussed. After storage for up to 12 months, CFU titers of bacteria (R. erythropolis, A. seifertii, P. aeruginosa, P. extremaustralis) and yeasts (Yarrovia lipolytica) immobilized in the gel based on silanol derivatives of humic substances were ten to one hundred times higher than in the control. After 4-month storage, hydrocarbon degradation by the stabilized cultures began earlier and was faster and more complete than in the control. The tested materials may be used to extend the storage time for the preparations of biotechnologically important bacteria and for application for petroleum bioremediation.

虽然用于水和土壤生物修复的液体生物制剂方便且具有经济吸引力，但微生物在标准环境条件下的存活率很差。微生物固定化是保持高活细胞滴度的常用且有效的方法。我们测试了三种新材料在长期储存期间对各种碳氢化合物氧化剂的存活率的影响。在评估长期储存后固定培养物的生存能力时，在相同条件下储存的悬浮（浮游）培养物用作对照。的细胞不动杆菌seifertii，假单胞菌extremaustralis，铜绿假单胞菌，红串红球菌，和迪茨氏马里斯用壳聚糖改性的聚脲微胶囊（MCs）（由皱巴巴的球体表示，直径 40-200 µm）生长，大量附着在 MC 表面。与 MC 一起储存几个月后，CFU 滴度比对照高 2 到 5 倍。用聚丙交酯 (PLA) MCs（由大小为 25-100 µm 的略微皱巴巴的薄壁球体表示）培养铜绿假单胞菌和R. erythropolis导致在 MC 表面形成厚生物膜。使用这些 MC 储存 30 天后，红球藻的CFU 滴度比对照高出一千倍。在 PLA MCs 存在下，两种培养物的呼吸速率比没有 MCs 的对照高 5 到 8 倍。正在讨论固定化细胞更好存活的原因。储存长达 12 个月后，细菌（R. erythropolis、A. seifertii、P. aeruginosa、P. extremaustralis）和酵母（Yarrovia lipolytica）的CFU 滴度) 固定在基于腐殖质的硅烷醇衍生物的凝胶中比对照高十到一百倍。储存 4 个月后，稳定培养物的碳氢化合物降解开始得更早，而且比对照更快、更完全。被测材料可用于延长生物技术重要细菌制剂的储存时间和石油生物修复应用。