Pseudorabies (Aujeszky disease) virus (PRV) was eliminated from domestic swine in many countries using glycoprotein E (gE)-deleted vaccines and serum antibody gE ELISAs, but PRV continues to circulate in some regions and in most feral swine populations in the world. We created a dual-matrix (serum and oral fluid) indirect IgG gE ELISA (iELISA) and evaluated its performance using samples from 4 groups of 10 pigs each: negative control (NC), vaccination (MLV), PRV inoculation (PRV), and vaccination followed by challenge (MLV-PRV). All serum and oral fluid samples collected before PRV challenge and all NC samples throughout the study were negative for gE antibodies by commercial blocking ELISA (bELISA) and our iELISA. Nasal swab samples from 9 of 10 animals in the PRV group were gB quantitative PRC (qPCR) positive at 2 days post-inoculation (dpi). The oral fluid iELISA detected a significant S/P response in the PRV (p = 0.03) and MLV-PRV (p = 0.01) groups by 6 dpi. ROC analyses of serum bELISA (n = 428), serum iELISA (n = 426), and oral fluid iELISA (n = 247) showed no significant differences in performance (p > 0.05). Our data support the concept of PRV surveillance based on oral fluid samples tested by an indirect gE ELISA.

许多国家使用缺失糖蛋白 E (gE) 的疫苗和血清抗体 gE ELISA 从家猪中消除了伪狂犬病（Aujeszky 病）病毒 (PRV)，但 PRV 继续在某些地区和世界上大多数野猪种群中传播。我们创建了双基质（血清和口腔液）间接 IgG gE ELISA (iELISA)，并使用来自 4 组每组 10 头猪的样本评估其性能：阴性对照 (NC)、疫苗接种 (MLV)、PRV 接种 (PRV)、和接种后攻击（MLV-PRV）。在 PRV 攻击前收集的所有血清和口腔液样品以及整个研究中的所有 NC 样品通过商业阻断 ELISA (bELISA) 和我们的 iELISA 对 gE 抗体呈阴性。PRV 组 10 只动物中有 9 只鼻拭子样本在接种后 2 天 (dpi) 呈 gB 定量 PRC (qPCR) 阳性。p = 0.03) 和 MLV-PRV ( p = 0.01) 按 6 dpi 分组。血清 bELISA ( n = 428)、血清 iELISA ( n = 426) 和口腔液 iELISA ( n = 247) 的 ROC 分析显示性能无显着差异 ( p > 0.05)。我们的数据支持基于间接 gE ELISA 测试的口腔液样本的 PRV 监测概念。