3-chloropropane-1,2-diol (3-MCPD) and its toxic metabolite glycidol were classified by the International Agency for Research on Cancer (IARC) as belonging to group 2B and 2A for humans. This study aimed to determine the sub-acute toxicity of these agents. Rats were exposed to 3-MCPD at 0.87 and 10 mg/kg/bw and glycidol (2,4 and 37,5 mg/kg/bw) for 90 days. miR-21 gene expression levels significantly decreased in all group’s cerebellar tissues compared with control. Exposure to 10 mg/kg/bw 3-MCPD showed significant increases in PTEN in brain as compared to control group. The Akt gen expressions were significantly decreased in 3-MCPD and glycidol groups when compared to control group brains. Additionally, Caspase 3 and AIF immunopositivity significantly increased in 3-MCPD high dose and glycidol high dose groups in cerebellum granular layers compared to control. The results of the present study conclude that 3-MCPD and glycidol can induce apoptosis in rat brain tissue.

3-氯丙烷-1,2-二醇 (3-MCPD) 及其有毒代谢物缩水甘油被国际癌症研究机构 (IARC) 分类为人类 2B 和 2A 组。本研究旨在确定这些药物的亚急性毒性。大鼠暴露于 0.87 和 10 mg/kg/bw 的 3-MCPD 以及缩水甘油（2,4 和 37,5 mg/kg/bw）90 天。与对照组相比，所有组小脑组织中 miR-21 基因表达水平均显着降低。与对照组相比，暴露于 10 mg/kg/bw 3-MCPD 后大脑中 PTEN 显着增加。与对照组大脑相比，3-MCPD 和缩水甘油组的 Akt 基因表达显着降低。此外，与对照组相比，小脑颗粒层中 3-MCPD 高剂量组和缩水甘油高剂量组的 Caspase 3 和 AIF 免疫阳性显着增加。本研究结果得出结论，3-MCPD和缩水甘油可以诱导大鼠脑组织细胞凋亡。