A label-free ratiometric method to detect Hg2+ based on structural change of DNA,Luminescence

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A label-free ratiometric method to detect Hg2+ based on structural change of DNA
Luminescence ( IF 3.2 ) Pub Date : 2021-08-25 , DOI: 10.1002/bio.4134
Mingming Xu ₁ , Yu Peng ₁ , Huan Liu ₁ , Xinyu Tian ₁ , Hualin Yang _{1,

2} , Yu Zhou _{1,

3}

Affiliation

In this work, a simple ratiometric method has been designed to detect Hg²⁺ based on the structural change between double-stranded DNA (dsDNA) and its G-quadruplex structure. When Hg²⁺ was added, the designed G-quadruplex structure could change into the corresponding dsDNA by forming the T–Hg²⁺–T mismatch. This kind of variation resulted in a decrease in the fluorescence of the G-quadruplex/N-methyl mesoporphyrin IX (NMM) complex and an increase in the fluorescence from the dsDNA/SYBR Green I (SG I) pair. The secondary excitation wavelength of SG I was used to excite NMM and SG I simultaneously. The titration experiment indicated that the new method had a linear response within 0.7–2.5 μM Hg²⁺ with a limit of detection of 9.3 nM. Because using the T–Hg²⁺–T mismatch to recognize Hg²⁺ was very specific, the selectivity of the new method was also satisfactory. The recoveries ranged from 92.8% to 110.2% suggested that this new method could achieve a potential application for Hg²⁺ detection in real environmental samples.

中文翻译：

一种基于DNA结构变化的无标记比率检测Hg2+方法

在这项工作中，基于双链 DNA (dsDNA) 与其 G-四链体结构之间的结构变化，设计了一种简单的比率方法来检测 Hg ²⁺。当添加Hg ²⁺时，设计的 G-四链体结构可以通过形成 T–Hg ²⁺ –T 错配而转变为相应的 dsDNA 。这种变异导致 G-四链体/ N-甲基中卟啉 IX (NMM) 复合物的荧光降低，而 dsDNA/SYBR Green I (SG I) 对的荧光增加。SG I 的二次激发波长用于同时激发 NMM 和 SG I。滴定实验表明，新方法在 0.7-2.5 μM Hg ²⁺范围内具有线性响应检测限为 9.3 nM。由于使用 T–Hg ²⁺ –T 错配识别 Hg ²⁺具有很高的特异性，因此新方法的选择性也令人满意。回收率从 92.8% 到 110.2% 不等，表明这种新方法可以实现在实际环境样品中检测Hg ²⁺的潜在应用。

更新日期：2021-08-25

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