This study aims to compare the effects of different carbon sources on sulfur-oxidizing denitrifying microorganisms by using glucose, ethanol, and acetate as carbon sources. Under the same chemical oxygen demand Cr (COD_Cr), nitrate, and sulfide concentrations, the removal rate of nitrate and total organic carbon, and the yield of elemental sulfur in a static experiment and a continuous flow reactor with glucose as the carbon source were lower than those with ethanol and acetic acid as the carbon source. The core sulfur-oxidizing denitrifying bacteria that use glucose as the carbon source were Azoarcus, Geoalkalibacter, and Mangroviflexus; those that use ethanol as the carbon source were Arcobacter, Pseudomonas, and Thauera; those that use acetate as the carbon source were Pseudomonas and Azoarcus. The metabolic activity of microorganisms that use different carbon sources was explained by functional gene detection. The fluctuation of gltA, a functional gene indicating heterotrophic metabolism of microorganisms, was small in three reactors, but that of the sulfur oxidation gene, Sqr, in the reactor with acetic acid as the carbon source was larger. Our results suggest that acetate is a more suitable carbon source for denitrification-desulfurization systems.

本研究旨在以葡萄糖、乙醇和醋酸盐为碳源，比较不同碳源对硫氧化反硝化微生物的影响。在相同化学需氧量Cr（COD _Cr）、硝酸盐和硫化物浓度下，以葡萄糖为碳源的静态实验和连续流动反应器中硝酸盐和总有机碳的去除率以及元素硫的收率分别为低于以乙醇和乙酸为碳源的。以葡萄糖为碳源的硫氧化反硝化菌的核心是Azoarcus、Geoalkalibacter和Mangroviflexus；那些使用乙醇作为碳源的是Arcobacter ,假单胞菌属和陶氏菌属；以醋酸盐为碳源的有假单胞菌属和固氮菌属。通过功能基因检测解释了使用不同碳源的微生物的代谢活动。的波动的gltA，功能性基因指示微生物的异养代谢，小在三个反应器中，但硫氧化基因，那SQR，在用乙酸作为碳源是大的反应器中。我们的研究结果表明，醋酸盐是更适合脱硝-脱硫系统的碳源。