Paper-based microfluidic analytical devices (μPADs) have shown great potential in the field of analysis due to their advantages of rapid analysis, environmental friendliness and the ability to realize the flow of fluid without external power. Saliva is an emerging biofluid which is used in diseases diagnostic and screening for the easy collection and the reflection of the physiological state. This review focuses on the fabrication methods for two-dimensional (2D) and three-dimensional (3D) μPADs and their applications on the saliva analysis. In the first part, the flow mechanism in μPADs is discussed. The second part mainly introduces the fabrication methods for the μPADs and compares the different methods. The third part presents the application of μPADs in the detection of biomarkers such as nitrite, glucose, and thiocyanate in saliva. Finally, the research directions of saliva analysis are discussed in the conclusion. There have been a lot of researches on μPADs, but the fabrication methods and applications need to be further studied to meet the commercial needs.

纸基微流体分析装置（μPADs）由于其分析速度快、环境友好以及无需外部动力即可实现流体流动的优势，在分析领域显示出巨大的潜力。唾液是一种新兴的生物流体，用于疾病诊断和筛查，易于收集和反映生理状态。本综述重点介绍二维 (2D) 和三维 (3D) μPAD 的制造方法及其在唾液分析中的应用。在第一部分中，讨论了 μPAD 中的流动机制。第二部分主要介绍μPADs的制作方法并比较不同的制作方法。第三部分介绍了μPADs在唾液中亚硝酸盐、葡萄糖和硫氰酸盐等生物标志物检测中的应用。最后，结论部分讨论了唾液分析的研究方向。关于μPADs的研究很多，但其制备方法和应用需要进一步研究以满足商业需求。