In this work, several immobilization strategies for Gluconobacter oxydans NBRC 14819 (Gox) were tested in the bioconversion of crude glycerol to dihydroxyacetone (DHA). Agar immobilized Gox proved to be the best heterogeneous biocatalyst reaching quantitative conversions of crude glycerol (50 g/L) to DHA solely in water. Immobilization allowed reutilization for at least 8 cycles, reaching 4 times more DHA (202 ± 5 g/L) than the amount obtained by free cells. An increase in scale of 34 times had no impact on DHA productivity. The results obtained herein constitute a contribution to the microbiological production of DHA as they not only attain unprecedented productivities for the reaction with immobilized biocatalysts but also proved that it is feasible to do it in a clean background of solely water that alleviates the cost of downstream processing.

在这项工作中，氧化葡糖杆菌NBRC 14819 (Gox) 的几种固定策略在粗甘油到二羟基丙酮 (DHA) 的生物转化中进行了测试。琼脂固定化 Gox 被证明是最好的非均相生物催化剂 ，仅在水中即可将粗甘油 (50 g/L)定量转化为 DHA。固定允许重复利用至少 8 个循环，达到 4 倍以上的 DHA (202 ± 5 g/L) 比游离细胞获得的量。规模增加 34 倍对 DHA 生产力没有影响。本文获得的结果对 DHA 的微生物生产做出了贡献，因为它们不仅在与固定化生物催化剂的反应中获得了前所未有的生产力，而且还证明在纯水的清洁背景下进行反应是可行的，从而降低了下游加工的成本.