The outbreak of African swine fever (ASF) has caused significant economic losses to animal husbandry worldwide. Currently, there is no effective vaccine or treatment available to control the disease, and therefore, efficient disease control is dependent on early detection and diagnosis of ASF virus (ASFV). In this study, a chemiluminescent immunoassay (CLIA) was developed using the ASFV protein p54 as a serum diagnostic antigen and an anti-p54 monoclonal antibody. After optimizing the working parameters of the CLIA, the sensitivity of the established CLIA was 1:128, ASFV-specific serum antibody was identified, and there was no cross-reaction with other swine virus antibodies. After testing 49 clinical serum samples, the consistency rate between the CLIA and the World Organization for Animal Health (OIE) recommended commercial kit was 100 %. Thus, this CLIA had a high degree of specificity, sensitivity, and reliability, and could be used as a rapid detection method for epidemiological investigations of ASFV infection.

非洲猪瘟（ASF）的爆发给全球畜牧业造成了重大经济损失。目前，还没有有效的疫苗或治疗方法来控制该疾病，因此，有效的疾病控制取决于 ASF 病毒 (ASFV) 的早期检测和诊断。在这项研究中，使用 ASFV 蛋白 p54 作为血清诊断抗原和抗 p54 单克隆抗体开发了一种化学发光免疫测定法 (CLIA)。优化CLIA工作参数后，建立的CLIA灵敏度为1:128，鉴定出ASFV特异性血清抗体，与其他猪病毒抗体无交叉反应。在对 49 份临床血清样本进行检测后，CLIA 与世界动物卫生组织 (OIE) 推荐的商业试剂盒的一致性率为 100%。因此，