Development of a Yeast-Based Assay for Bioavailable Phosphorus,ACS ES&T Water

当前位置： X-MOL 学术 › ACS ES&T Water › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Development of a Yeast-Based Assay for Bioavailable Phosphorus
ACS ES&T Water ( IF 4.8 ) Pub Date : 2021-08-26 , DOI: 10.1021/acsestwater.1c00111
Heather A. M. Shepherd ₁ , Matt T. Trentman _{2,

3} , Jennifer L. Tank _{2,

3} , Jennifer Praner ₁ , Anissa Cervantes ₁ , Priya Chaudhary _{1,

4} , Jonah Gezelter _{1,

5} , Allyson J. Marrs ₂ , Kathryn A. Myers _{1,

4} , Jonathan R. Welsh ₁ , Yueh-Fu O. Wu ₁ , Holly V. Goodson _{1,

2,

3}

Affiliation

Preventing the eutrophication of inland freshwater ecosystems requires quantifying the phosphorus (P) content of the streams and rivers that feed them. Typical methods for measuring P assess soluble reactive P (SRP) or total P (TP) and require expensive analytical techniques that produce hazardous waste. Here, we present a novel, low-tech method for measuring the more relevant bioavailable P (BAP); this assay utilizes the growth of baker’s yeast, avoids the production of hazardous waste, and reduces cost relative to SRP and TP measurements. The yeast BAP (yBAP) assay takes advantage of the observation that yeast density at saturating growth increases linearly with provided P. We show that this relationship can be used to measure P in freshwater in concentration ranges relevant to eutrophication. In addition, we measured yBAP in water containing known amounts of fertilizer and in samples from agricultural waterways. We observed that the majority of yBAP values were between those obtained from standard SRP and TP measurements, demonstrating that the assay is compatible with real-world settings. The cost-effective and nonhazardous nature of the yeast-based assay suggests that it could have utility in a range of settings, offering added insight into identify water systems at risk of eutrophication from excess phosphorus.

中文翻译：

基于酵母的生物可利用磷检测方法的开发

防止内陆淡水生态系统的富营养化需要量化供给它们的溪流和河流的磷 (P) 含量。测量 P 的典型方法评估可溶性活性 P (SRP) 或总 P (TP)，并且需要昂贵的分析技术，会产生危险废物。在这里，我们提出了一种新的、低技术的方法来测量更相关的生物可利用 P (BAP)；该测定利用面包酵母的生长，避免产生危险废物，并降低相对于 SRP 和 TP 测量的成本。酵母 BAP (yBAP) 测定利用观察到饱和生长时酵母密度随所提供的 P 线性增加。我们表明这种关系可用于测量淡水中与富营养化相关的浓度范围内的 P。此外，我们在含有已知量肥料的水中和来自农业水道的样品中测量了 yBAP。我们观察到大多数 yBAP 值介于从标准 SRP 和 TP 测量中获得的值之间，这表明该测定与实际设置兼容。基于酵母的测定具有成本效益和无害的性质，表明它可以在一系列环境中发挥作用，为识别存在过量磷富营养化风险的水系统提供更多见解。

更新日期：2021-09-10

点击分享查看原文

点击收藏

阅读更多本刊最新论文