The aim of this study was to explore the correlation between intraoperative hyperalgesia of the second eye and the dynamic changes of tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels in aqueous humor (AH) of the second eye and whole blood after the first eye cataract surgery. A rabbit model of monocular phacoemulsification was established by administration of 0.3% levofloxacin. Whole blood and AH samples from non-surgical eyes in the experimental group (n =25) and second eye in the blank control group (n =15) were obtained and corneal sensitivity was examined after surgery (1, 3, 7, 14, and 21 days postoperatively). TNF-α and IL-1β levels in AH and TNF-α mRNA and IL-1β mRNA levels in whole blood were measured. In a clinical study, 30 patients who underwent bilateral phacoemulsification within 1 month were divided into six groups in accordance with the operation intervals (1, 3, 7, 10, 14, and 21days). TNF-α and IL-1β levels in AH were measured at the beginning of surgery and intraoperative pain was assessed immediately after surgery. Corneal sensitivity (F =244.910, P <0.05), TNF-α and IL-1β levels in AH (F =184.200, 82.900, P <0.05) of non-surgical eyes and in whole blood (F =272.800, 193.530, P <0.05) in the experimental group were significantly higher than the baseline levels after phacoemulsification. In the clinical study, NRS scores of second eye surgery were higher than those of the first eye(P =0.0025) and 19 (63.3%) patients reported more pain during the second eye surgery. TNF-α and IL-1β concentrations in AH of the second eye were significantly higher than those of the first eye (F =123.60, P <0.05; F =59.60, P <0.05). In conclusion, within 1 month after the first eye phacoemulsification, higher pain sensitivity (hyperalgesia) exists in the second eye, which may be related to dynamic changes in TNF-α, IL-1β levels in AH or whole blood.

中文翻译：

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第二眼超声乳化术中痛觉过敏与房水和血液中 TNF-α 和 IL-1β 上调的相关性：临床和实验研究

本研究旨在探讨第二只眼术中痛觉过敏与第二只眼房水（AH）中肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β水平动态变化之间的相关性，以及第一次眼白内障手术后的全血。给予0.3%左氧氟沙星建立兔单眼超声乳化模型。实验组（n = 25）和空白对照组的第二只眼（n = 15）获得全血和AH样本，并在手术后检查角膜敏感性（1, 3, 7, 14,和术后 21 天）。AH 中的 TNF-α 和 IL-1β 水平以及 TNF - α mRNA和IL - 1β测量全血中的mRNA水平。在一项临床研究中，将 30 名在 1 个月内接受双侧超声乳化术的患者按照手术间隔（1、3、7、10、14 和 21 天）分为 6 组。在手术开始时测量 AH 中的TNF - α和 IL-1 β水平，并在手术后立即评估术中疼痛。角膜敏感性（F = 244.910，P <0.05），TNF - α和 IL-1 β非手术眼AH（F=184.200、82.900、P<0.05）和实验组全血（F=272.800、193.530、P<0.05）超声乳化术后明显高于基线水平。在临床研究中，第二眼手术的NRS评分高于第一眼（P = 0.0025），19名（63.3％）患者在第二眼手术期间报告了更多的疼痛。第二眼AH中TNF - α和IL- 1β浓度显着高于第一眼（F=123.60，P<0.05；F=59.60，P<0.05）。综上所述，第一只眼超声乳化术后1个月内，第二只眼出现较高的疼痛敏感性（痛觉过敏），这可能与TNF - α、IL-1β的动态变化有关。 AH 或全血中的水平。