Abstract

2,3-Butanediol (2,3-BD) has been extensively used in chemical syntheses. This study aimed to explore acetic acid as a signaling molecule that activates a quorum sensing (QS) system to promote the production of 2,3-BD. The yield of 2,3-BD is proportional to the cell density. Saccharomyces cerevisiae W141 does not produce 2,3-BD when the cell density is lower than the threshold concentration (OD_600 nm = 10 or cell density 4.4 × 10⁸ CFU/mL). When 1.5 g/L acetic acid is added, the yield of 2,3-BD is 3.01 ± 0.04 g/L. Subsequently, S. cerevisiae W141 was cocultured with Acetobacter pasteurianus Huniang 1.01 under the optimal conditions, the acetic acid production was increased by 76.7% and 30.6% compared with the original strain and the strain cultivated with 1.5 g/L acetic acid, and the yield of 2,3-BD was increased by 81.9% and 3.3%, respectively. This difference is due to the activity of acetyl lactic acid synthase (ILV2) and 2,3-BD dehydrogenase (BDH1), as the relative expression of the ilv2 and bdh1 genes is increased. The results showed that the biosynthesis of 2,3-BD was regulated by acetic acid as a signaling molecule. S. cerevisiae is a promising host for producing 2,3-BD for industrial applications.

摘要

2,3-丁二醇 (2,3-BD) 已广泛用于化学合成。本研究旨在探索乙酸作为激活群体感应 (QS) 系统以促进 2,3-BD 产生的信号分子。2,3-BD 的产量与细胞密度成正比。当细胞密度低于阈值浓度（OD _{600 nm} = 10 或细胞密度 4.4 × 10 ⁸ CFU/mL）时，酿酒酵母W141 不会产生 2,3-BD。添加1.5 g/L乙酸时，2,3-BD的收率为3.01±0.04 g/L。随后，S. cerevisiae W141 与巴氏醋杆菌共培养优化条件下的湘乡1.01，醋酸产量比原菌株和1.5 g/L醋酸培养的菌株分别提高了76.7%和30.6%，2,3-BD的产量提高了81.9%和 3.3%，分别。这种差异是由于乙酰乳酸合酶 (ILV2) 和 2,3-BD 脱氢酶 (BDH1) 的活性，因为ilv2和bdh1基因的相对表达增加。结果表明，2,3-BD的生物合成受乙酸作为信号分子的调控。S. cerevisiae是生产用于工业应用的 2,3-BD 的有前途的宿主。