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Exogenous Application of Methionine and Phenylalanine Confers Salinity Tolerance in Tomato by Concerted Regulation of Metabolites and Antioxidants
Journal of Soil Science and Plant Nutrition ( IF 3.4 ) Pub Date : 2021-08-25 , DOI: 10.1007/s42729-021-00588-9
Hafiza Iqra Almas 1 , Zaib -un-Nisa 2 , Sumera Anwar 2 , Abida Kausar 3 , Fozia Farhat 3 , Muneeb Munawar 4 , Raziah Khalizadieh 5
Affiliation  

A pot experiment was carried out to study physiological and yield attributes of tomato by foliar spray of L-methionine (Met) and L-phenylalanine (Phe) under saline stress (0, 4, and 6 dS/m). The tomato plants were sprayed with Met (0.01% and 0.02%), Phe (0.01% and 0.02%), and their combinations (0.01% Met + 0.01% Phe and 0.02% Met + 0.02% Phe) at the vegetative growth stage. The results showed that 0.02% Met concentration significantly improved all the growth and yield parameters like shoot length by 25.7%, plant fresh biomass by 66.89%, fruit weight by 67.26%, dry biomass by 37%, and the number of fruits by 82% under 6 dS/m stress condition. Plants sprayed with 0.02% Met + 0.02% Phe displayed higher carotenoid contents (30%), total carbohydrates (threefold), total free amino acids (53%), and proline contents (82%) compared to only salt-treated plants. Also, the combined application of both amino acids reduced the lipid peroxidation rate in a range of 54–57% and electrolyte leakage by 62% compared to salinity alone. The antioxidative activity of peroxidase and catalase was decreased by foliar application under salinity. These results suggest that foliar application of Met and Phe effectively alleviated the harmful effects of salinity on the growth and yield of tomato plants by enhancing the plasma membrane stability, increased osmolytes contents, and activity of antioxidative enzymes. Thus, the foliar spray of methionine can induce salt resistance and increase tomato yield.



中文翻译:

外源应用蛋氨酸和苯丙氨酸通过代谢物和抗氧化剂的协同调节赋予番茄耐盐性

通过盆栽试验研究了在盐胁迫(0、4 和 6 dS/m)下叶面喷洒 L-蛋氨酸 (Met) 和 L-苯丙氨酸 (Phe) 的番茄生理和产量特性。在营养生长阶段用 Met(0.01% 和 0.02%)、Phe(0.01% 和 0.02%)及其组合(0.01% Met + 0.01% Phe 和 0.02% Met + 0.02% Phe)喷洒番茄植株。结果表明,0.02% Met 浓度显着提高了所有生长和产量参数,如枝条长度提高 25.7%,植物新鲜生物量提高 66.89%,果实重量提高 67.26%,干生物量提高 37%,果实数量提高 82%在 6 dS/m 应力条件下。喷洒 0.02% Met + 0.02% Phe 的植物表现出更高的类胡萝卜素含量 (30%)、总碳水化合物 (三倍)、总游离氨基酸 (53%)、和脯氨酸含量 (82%) 与仅盐处理的植物相比。此外,与单独使用盐分相比,两种氨基酸的联合应用降低了 54-57% 的脂质过氧化率和 62% 的电解质泄漏。盐度下叶面喷施降低过氧化物酶和过氧化氢酶的抗氧化活性。这些结果表明,叶面喷施 Met 和 Phe 通过增强质膜稳定性、增加渗透物含量和抗氧化酶的活性,有效地减轻了盐分对番茄植株生长和产量的有害影响。因此,叶面喷施蛋氨酸可以诱导抗盐性并增加番茄产量。与单独使用盐分相比,两种氨基酸的联合应用将脂质过氧化率降低了 54-57%,电解质泄漏降低了 62%。盐度下叶面喷施降低过氧化物酶和过氧化氢酶的抗氧化活性。这些结果表明,叶面喷施 Met 和 Phe 通过增强质膜稳定性、增加渗透物含量和抗氧化酶的活性,有效地减轻了盐分对番茄植株生长和产量的有害影响。因此,叶面喷施蛋氨酸可以诱导抗盐性并增加番茄产量。与单独使用盐分相比,两种氨基酸的联合应用将脂质过氧化率降低了 54-57%,电解质泄漏降低了 62%。盐度下叶面喷施降低过氧化物酶和过氧化氢酶的抗氧化活性。这些结果表明,叶面喷施 Met 和 Phe 通过增强质膜稳定性、增加渗透物含量和抗氧化酶的活性,有效地减轻了盐分对番茄植株生长和产量的有害影响。因此,叶面喷施蛋氨酸可以诱导抗盐性并增加番茄产量。这些结果表明,叶面喷施 Met 和 Phe 通过增强质膜稳定性、增加渗透物含量和抗氧化酶的活性,有效地减轻了盐分对番茄植株生长和产量的有害影响。因此,叶面喷施蛋氨酸可以诱导抗盐性并增加番茄产量。这些结果表明,叶面喷施 Met 和 Phe 通过增强质膜稳定性、增加渗透物含量和抗氧化酶的活性,有效地减轻了盐分对番茄植株生长和产量的有害影响。因此,叶面喷施蛋氨酸可以诱导抗盐性并增加番茄产量。

更新日期:2021-08-26
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