Abstract

In this research article, we present a simple and rapid method for synthesis of zinc oxide nanoparticles (ZnONPs) using zinc acetate dehydrate, sodium hydroxide and leaf extract of an important anti-diabetic plant Andrographis serpelifolia (As). The results reveal that UV-visible absorption spectrum indicates peaks at 325 nm which confirms the biosynthesis of ZnONPs. Fourier transform infrared spectroscopy (FT-IR) results indicate the characteristic peak at 511.93 cm⁻¹ for ZnO nanoparticles. EDX analysis revealed the purity of As-ZnONPs and the particle size was between 70 nm and 80 ± 10 nm by both dynamic light scattering analysis (DLS) and Transmission electron microscopy (TEM) analysis. The As-ZnONPs were moderately stable with negative zeta potential value −11.1 mV. Atomic force microscopy (AFM) analysis also revealed the same results as TEM and DLS. The x-ray diffraction (XRD) data revealed that the particles are facets of face-centered cubic crystal structure of ZnO. The As-ZnONPs have exhibited potential antioxidant activity and effective antibacterial activity against gram-positive bacteria. The As-ZnONPs have also potential anti-diabetic activity in vivo (rat model). The antidiabetic activity of As-ZnONPs comparable to that of the standard drug glibenclamide was evidenced by various biochemical parameters like HbA1C, serum lipid profiles, liver and renal functional markers.

摘要

在这篇研究文章中，我们提出了一种使用脱水醋酸锌、氢氧化钠和重要的抗糖尿病植物穿心莲(As)的叶子提取物合成氧化锌纳米粒子 (ZnONPs) 的简单快速的方法。结果表明，紫外-可见吸收光谱在 325 nm 处有峰，这证实了 ZnONPs 的生物合成。傅里叶变换红外光谱 (FT-IR) 结果表明特征峰位于 511.93 cm ^-1用于 ZnO 纳米粒子。EDX 分析通过动态光散射分析 (DLS) 和透射电子显微镜 (TEM) 分析显示 As-ZnONPs 的纯度和粒径在 70 nm 和 80 ± 10 nm 之间。As-ZnONPs 适度稳定，负 zeta 电位值为 -11.1 mV。原子力显微镜 (AFM) 分析也揭示了与 TEM 和 DLS 相同的结果。X射线衍射(XRD)数据表明，这些颗粒是ZnO的面心立方晶体结构的晶面。As-ZnONPs 对革兰氏阳性菌具有潜在的抗氧化活性和有效的抗菌活性。As-ZnONPs在体内也具有潜在的抗糖尿病活性（大鼠模型）。As-ZnONPs 的抗糖尿病活性与标准药物格列本脲的抗糖尿病活性相当，可通过 HbA1C、血清脂质谱、肝肾功能标志物等各种生化参数得到证实。