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A multi-channel handheld automatic spectrometer for wide range and on-site detection of okadaic acid based on specific aptamer binding
Analytical Methods ( IF 2.7 ) Pub Date : 2021-08-03 , DOI: 10.1039/d1ay00976a Chiyu Ma 1 , Xianyou Sun 1 , Liubing Kong 1 , Xinyi Wang 1 , Shuqi Zhou 1 , Xinwei Wei 1 , Dmitry Kirsanov 2 , Andrey Legin 2 , Hao Wan 1 , Ping Wang 1
Analytical Methods ( IF 2.7 ) Pub Date : 2021-08-03 , DOI: 10.1039/d1ay00976a Chiyu Ma 1 , Xianyou Sun 1 , Liubing Kong 1 , Xinyi Wang 1 , Shuqi Zhou 1 , Xinwei Wei 1 , Dmitry Kirsanov 2 , Andrey Legin 2 , Hao Wan 1 , Ping Wang 1
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Okadaic acid (OA) is one of the marine toxins that are widely distributed and harmful to humans. However, the current detection methods for OA involve complex procedures, need long detection time, and rely on large-scale laboratory equipment. In this work, a multi-channel handheld automatic spectrometer (MHAS) based on a spectral sensor was developed with the advantages of small size, simple operation and low cost. It could achieve rapid detection within 30 s and a wide spectral detection range of 470–780 nm with a broadband LED as the light source and a microplate containing 8 wells as a sample cell. Moreover, through the combination of gold nanoparticles (AuNPs) and aptamer-OA34, a highly sensitive and rapid system for OA detection was established with a LOD of 1.80 μg L−1 and a wide detection range of 20–10 000 μg L−1, which is comparable to a microplate reader. Compared with other studies, the proposed MHAS realized rapid on-site detection of OA with a wider detection range, shorter detection time and higher portability. Therefore, the MHAS promises to be a stable and efficient optical detection instrument for on-site detection in the fields of food safety, disease diagnosis and environmental monitoring.
中文翻译：
基于特异性适体结合的冈田酸大范围现场检测多通道手持式自动光谱仪
冈田酸（OA）是一种分布广泛且对人类有害的海洋毒素。但目前OA的检测方法流程复杂，检测时间长，且依赖大型实验室设备。在这项工作中，开发了一种基于光谱传感器的多通道手持式自动光谱仪（MHAS），具有体积小、操作简单、成本低的优点。以宽带LED为光源，以8孔微孔板为样品池，可实现30s内的快速检测和470-780nm的宽光谱检测范围。此外，通过金纳米粒子（AuNPs）和适配体-OA34的结合，建立了一个高度灵敏、快速的OA检测系统，LOD为1.80 μg L -1以及 20–10 000 μg L -1的宽检测范围，可与酶标仪相媲美。与其他研究相比，所提出的MHAS实现了OA的快速现场检测，具有更广的检测范围、更短的检测时间和更高的便携性。因此，MHAS有望成为食品安全、疾病诊断和环境监测领域现场检测的稳定高效的光学检测仪器。
更新日期：2021-08-26
中文翻译：
基于特异性适体结合的冈田酸大范围现场检测多通道手持式自动光谱仪
冈田酸（OA）是一种分布广泛且对人类有害的海洋毒素。但目前OA的检测方法流程复杂，检测时间长，且依赖大型实验室设备。在这项工作中，开发了一种基于光谱传感器的多通道手持式自动光谱仪（MHAS），具有体积小、操作简单、成本低的优点。以宽带LED为光源，以8孔微孔板为样品池，可实现30s内的快速检测和470-780nm的宽光谱检测范围。此外，通过金纳米粒子（AuNPs）和适配体-OA34的结合，建立了一个高度灵敏、快速的OA检测系统，LOD为1.80 μg L -1以及 20–10 000 μg L -1的宽检测范围，可与酶标仪相媲美。与其他研究相比，所提出的MHAS实现了OA的快速现场检测，具有更广的检测范围、更短的检测时间和更高的便携性。因此，MHAS有望成为食品安全、疾病诊断和环境监测领域现场检测的稳定高效的光学检测仪器。
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