Previous studies have shown that lysine-specific demethylase 1 (LSD1) could regulate cell cycle progression through demethylation. The 3′domain of HOX transcript antisense RNA (Hotair) combined with the LSD1/CoREST/REST complex helps LSD1 target the corresponding gene. However, its role in mice's myocardial regeneration is still unclear. The heart from neonatal mice shows strong myocardial regeneration ability, but this ability disappears 7 days after birth. Our study shows that the myocardial tissue highly expresses Hotair and Lsd1 within 1 week after birth, consistent with the myocardial regeneration time window. Knockdown Lsd1 or Hotair expression by RNA interference could inhibit myocardial regeneration and cardiomyocyte proliferation. Our results suggest that Hotair-mediated demethylation of LSD1 may play an important role in myocardial regeneration in neonatal mice.

中文翻译：

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Hotair 或 LSD1 的下调会损害新生小鼠的心脏再生

先前的研究表明，赖氨酸特异性去甲基化酶 1 (LSD1) 可以通过去甲基化调节细胞周期进程。HOX 转录反义 RNA ( Hotair) 的 3' 结构域与 LSD1/CoREST/REST 复合物结合帮助 LSD1 靶向相应基因。然而，其在小鼠心肌再生中的作用尚不清楚。新生小鼠的心脏显示出很强的心肌再生能力，但这种能力在出生后7天就消失了。我们的研究表明，心肌组织在出生后 1 周内高表达Hotair和Lsd1，与心肌再生时间窗口一致。击倒Lsd1或HotairRNA干扰表达可抑制心肌再生和心肌细胞增殖。我们的结果表明Hotair介导的 LSD1 去甲基化可能在新生小鼠的心肌再生中起重要作用。