Comparison on binding interactions of quercetin and its metal complexes with calf thymus DNA by spectroscopic techniques and viscosity measurement,Journal of Molecular Recognition

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Comparison on binding interactions of quercetin and its metal complexes with calf thymus DNA by spectroscopic techniques and viscosity measurement
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2021-08-25 , DOI: 10.1002/jmr.2933
Huajian Luo ₁ , Yu Liang ₁ , Huiying Zhang ₂ , Yi Liu _{1,

3} , Qi Xiao ₁ , Shan Huang ₁

Affiliation

Quercetin (Qu) and its metal complexes have received great attention during the last years, due to their good antioxidant, antibacterial, and anticancer activities. In this contribution, binding interactions of Qu and Qu–metal complexes with calf thymus DNA (ctDNA) were investigated and compared systematically by using spectroscopic techniques and viscosity measurement. UV-vis absorption spectra of ctDNA–compound systems showed obvious hypochromic effect. Relative viscosity and melting temperature of ctDNA increased after the addition of Qu and Qu–metal complexes, and the change tendency is Qu–Cr(III) > Qu–Mn(II) > Qu–Zn(II) > Qu–Cu(II) > Qu. Fluorescence competition experiments show that hydrogen bonds and van der Waals interaction play an important role in the intercalative binding of Qu and Qu–metal complexes with ctDNA. Qu and Qu–metal complexes could unwind the right-handed B-form helicity of ctDNA and further affect its base pair stacking. Space steric hindrance might be responsible for the differences in the intercalative binding between ctDNA and different Qu–metal complexes. These results provide new information for the molecular understanding of binding interactions of Qu–metal complexes with DNA and the strategy for research of structural influences.

中文翻译：

光谱技术和粘度测量比较槲皮素及其金属配合物与小牛胸腺 DNA 的结合相互作用

槲皮素 (Qu) 及其金属配合物由于其良好的抗氧化、抗菌和抗癌活性，在过去几年受到了极大的关注。在此贡献中，通过使用光谱技术和粘度测量系统地研究和比较了 Qu 和 Qu-金属配合物与小牛胸腺 DNA (ctDNA) 的结合相互作用。ctDNA-化合物体系的紫外-可见吸收光谱表现出明显的减色效应。加入Qu和Qu-金属配合物后ctDNA的相对黏度和熔解温度升高，变化趋势为Qu-Cr(III) > Qu-Mn(II) > Qu-Zn(II) > Qu-Cu(II) )>曲。荧光竞争实验表明，氢键和范德华相互作用在 Qu 和 Qu-金属配合物与 ctDNA 的嵌入结合中起着重要作用。Qu 和 Qu-金属配合物可以解开 ctDNA 的右手 B 型螺旋，并进一步影响其碱基对堆叠。空间位阻可能是造成 ctDNA 与不同 Qu-金属配合物之间嵌入结合差异的原因。这些结果为分子理解 Qu-金属配合物与 DNA 的结合相互作用和结构影响研究策略提供了新的信息。

更新日期：2021-10-08

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