Targeted proteomics is an attractive approach for the analysis of blood proteins. Here, we describe a novel analytical platform based on isotope-labeled recombinant protein standards stored in a chaotropic agent and subsequently dried down to allow storage at ambient temperature. This enables a straightforward protocol suitable for robotic workstations. Plasma samples to be analyzed are simply added to the dried pellet followed by enzymatic treatment and mass spectrometry analysis. Here, we show that this approach can be used to precisely (coefficient of variation <10%) determine the absolute concentrations in human plasma of hundred clinically relevant protein targets, spanning four orders of magnitude, using simultaneous analysis of 292 peptides. The use of this next-generation analytical platform for high-throughput clinical proteome profiling is discussed.

中文翻译：

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使用重组蛋白标准品对血浆蛋白进行靶向蛋白质组学分析，仅用于添加工作流程。

靶向蛋白质组学是分析血液蛋白质的一种有吸引力的方法。在这里，我们描述了一种基于同位素标记的重组蛋白标准品的新型分析平台，该标准品储存在离液剂中，随后干燥以允许在环境温度下储存。这使得适用于机器人工作站的简单协议成为可能。将待分析的血浆样品简单地添加到干燥的颗粒中，然后进行酶处理和质谱分析。在这里，我们展示了这种方法可用于精确（变异系数 <10%）确定人体血浆中数百个临床相关蛋白质靶标的绝对浓度，跨越四个数量级，同时分析 292 种肽。