Sepsis is defined as an organ dysfunction syndrome and it has high mortality worldwide. This study analysed the proteome of serum from patients with sepsis to characterize the pathological mechanism and pathways involved in sepsis. A total of 59 patients with sepsis were enrolled for quantitative proteomic analysis. Weighted gene co-expression network analysis (WGCNA) was performed to construct a co-expression network specific to sepsis. Key regulatory modules that were detected were highly correlated with sepsis patients and related to multiple functional groups, including plasma lipoprotein particle remodeling, inflammatory response, and wound healing. Complement activation was significantly associated with sepsis-associated encephalopathy. Triglyceride/cholesterol homeostasis was found to be related to sepsis-associated acute kidney injury. Twelve hub proteins were identified, which might be predictive biomarkers of sepsis. External validation of the hub proteins showed their significantly differential expression in sepsis patients. This study identified that plasma lipoprotein processes played a crucial role in sepsis patients, that complement activation contributed to sepsis-associated encephalopathy, and that triglyceride/cholesterol homeostasis was associated with sepsis-associated acute kidney injury.

中文翻译：

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血清蛋白质组学揭示脓毒症中脂蛋白代谢紊乱。

脓毒症被定义为器官功能障碍综合征，在世界范围内死亡率很高。本研究分析了脓毒症患者血清的蛋白质组，以表征脓毒症的病理机制和途径。共招募了 59 名败血症患者进行定量蛋白质组学分析。进行加权基因共表达网络分析（WGCNA）以构建特定于败血症的共表达网络。检测到的关键调控模块与脓毒症患者高度相关，并与多个功能组相关，包括血浆脂蛋白颗粒重塑、炎症反应和伤口愈合。补体激活与败血症相关脑病显着相关。发现甘油三酯/胆固醇稳态与败血症相关的急性肾损伤有关。鉴定了 12 种枢纽蛋白，它们可能是败血症的预测生物标志物。枢纽蛋白的外部验证显示它们在败血症患者中的显着差异表达。该研究确定血浆脂蛋白过程在脓毒症患者中发挥关键作用，补体激活导致脓毒症相关脑病，甘油三酯/胆固醇稳态与脓毒症相关急性肾损伤相关。