Drosophila suzukii is an invasive fruit pest in Europe and America. Females lay eggs into mature fruit that larvae consume causing important losses. Sterile insect technique (SIT) is under development to control this pest. The efficiency of this technique relies on insect quality. However, during the process from mass production to field release, several steps may compromise insect quality and therefore SIT success. Shipment of sterile insects after irradiation is a key step of SIT programmes. Generally, insects are shipped as pupae and conditions during transport need to be adapted to prevent emergence before field release, while guaranteeing insect quality. To do so, transport is usually performed under low temperature, hypoxia or a combination of both. However, the impact of multiple stressors such as irradiation followed by chilling combined with hypoxia is poorly described and has not been studied in D. suzukii. Therefore, the aim of this study was to simulate a shipment of D. suzukii pupae (irradiated or not) under different conditions (chilling combined or not with hypoxia) for various durations, and to assess consequences on emerged adults. Irradiation followed by hypoxia and/or chilling only weakly altered emergence. However, 48 h of hypoxia without chilling altered the flight ability of flies whether or not they were irradiated. Conversely, when hypoxia was combined with chilling, flight ability remained similar to that of untreated flies. The use of chilling in combination with hypoxia for 48 h could be implemented as a transportation method for SIT programme on D. suzukii.

中文翻译：

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缺氧结合冷冻维持辐照果蝇的质量：模拟装运实验

铃木果蝇是欧洲和美洲的一种入侵性水果害虫。雌性将卵产入成熟的果实中，幼虫食用会造成重大损失。昆虫不育技术 (SIT) 正在开发中以控制这种害虫。这种技术的效率取决于昆虫的质量。然而，在从大规模生产到田间释放的过程中，几个步骤可能会影响昆虫的质量，从而影响 SIT 的成功。辐照后不育昆虫的运输是 SIT 计划的关键步骤。通常，昆虫以蛹的形式运输，运输过程中需要调整条件以防止在田间释放前出现，同时保证昆虫的质量。为此，运输通常在低温、缺氧或两者结合的情况下进行。然而，D.铃木. 因此，本研究的目的是模拟运输D.铃木蛹（辐照或未辐照）在不同条件下（冷冻或不与缺氧相结合）不同持续时间，并评估对出现的成虫的后果。辐照后的缺氧和/或冷却仅轻微改变了出现。然而，48 小时没有冷却的缺氧改变了苍蝇的飞行能力，无论它们是否被照射。相反，当缺氧与寒冷相结合时，飞行能力仍然与未经处理的苍蝇相似。48 h 低温结合缺氧可作为 SIT 项目的运输方式D.铃木.