Abstract

The article addresses the role of TNF-α, IL-2, IL-5 and IL-6 in the contractile activity of rat tracheal and bronchial smooth muscle contractions. The studies were carried out on isolated preparations using electrical stimulation of postganglionic nerves (frequency 30 stim/s, duration 0.5 ms, amplitude 20 V, stimulation duration 10 s). Two groups of animals were formed: experimental rats were sensitized with ovalbumin by its intraperitoneal (i.p.) administration that was repeated after 14 days. Further, the contractile activity of tracheal and bronchial smooth muscles was assessed after repeated ovalbumin administration and perfusion of interleukin solutions. It was found that TNF-α, IL-2, IL-5 and IL-6 lead to a pronounced increase in contractile responses of tracheal and bronchial smooth muscles both in control and ovalbumin-sensitized animals. Among the studied cytokines, the strongest constrictor effect was exerted by TNF-α and IL-6.

中文翻译：

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TNF-α、IL-2、IL-5和IL-6对大鼠气管和支气管平滑肌收缩的影响

摘要

该文章讨论了 TNF-α、IL-2、IL-5 和 IL-6 在大鼠气管和支气管平滑肌收缩的收缩活动中的作用。使用节后神经电刺激（频率 30 stim/s，持续时间 0.5 ms，振幅 20 V，刺激持续时间 10 s）对分离的制剂进行研究。形成两组动物：实验大鼠通过其腹膜内(ip)给药用卵清蛋白致敏，该给药在14天后重复。此外，在重复给予卵清蛋白和灌注白细胞介素溶液后，评估气管和支气管平滑肌的收缩活性。发现 TNF-α、IL-2、IL-5 和 IL-6 导致对照动物和卵清蛋白致敏动物的气管和支气管平滑肌的收缩反应显着增加。