Abstract

The purpose of this article was to fabricate the novel poly(t-butyl betaine carboxylate)-S-S-poly (1, 3-dioxan-2-one) nanoparticles (PCB-tBU-S-S-PDI NPs) and study their in vivo biosecurity. The poly(t-butyl betaine carboxylate) (PCB-tBU) segment was conjugated to the poly(1, 3-dioxan-2-one)(PDI) moity with a disulfide bond to obtain the copolymer PCB-tBU-S-S-PDI. Hydrogen nuclear magnetic resonance (¹H NMR) and Fourier transform infrared spectroscopy (FTIR) spectra were applied to study the structure of PCB-tBU-S-S-PDI. The cargo-free NPs were administrated to Sprague-Dawley (SD) rats by intraperitoneal injection every 3 days for 30 days. Then, the blood routine examination, blood biochemistry, and histologic slides of rat’s organs were carried out to monitor the in vivo biosecurity of cargo-free PCB-tBU-S-S-PDI NPs. ¹H NMR and FTIR spectra confirmed the successfully synthesis of PCB-tBU-S-S-PDI. The cargo-free NPs showed spherical morphology with an average of 139.8 ± 0.26 nm. The results of blood biochemistry and blood routine examination suggested that the cargo-free PCB-tBU-S-S-PDI NPs did not show any influence on the liver and renal functions of treated rats. Significantly, the physiological slides of treated rat’s organs did not show any physiological and pathological changes. These phenomena suggested that the PCB-tBU-S-S-PDI NPs had good biosecurity, and it could be used as a vehicle for antineoplastic drug delivery.

摘要

本文的目的是制备新型聚（叔丁基甜菜碱羧酸盐）-SS-聚（1, 3-dioxan-2-one）纳米颗粒（PCB-tBU-SS-PDI NPs）并研究其体内生物安全性。 . 聚（叔丁基甜菜碱羧酸酯）（PCB-tBU）链段通过二硫键与聚（1, 3-dioxan-2-one）（PDI）部分共轭，得到共聚物 PCB-tBU-SS-PDI . 氢核磁共振（¹H NMR）和傅里叶变换红外光谱（FTIR）光谱用于研究PCB-tBU-SS-PDI的结构。每 3 天通过腹膜内注射将无货物 NPs 施用于 Sprague-Dawley (SD) 大鼠，持续 30 天。然后，对大鼠器官的血常规检查、血液生化和组织学切片进行了监测，以监测无货物 PCB-tBU-SS-PDI NPs 的体内生物安全性。¹H NMR和FTIR光谱证实了PCB-tBU-SS-PDI的成功合成。无货物 NP 呈球形，平均为 139.8 ± 0.26 nm。血液生化和血常规检查结果表明，无货物 PCB-tBU-SS-PDI NPs 对治疗大​​鼠的肝肾功能没有任何影响。值得注意的是，处理过的大鼠器官的生理载玻片没有显示出任何生理和病理变化。这些现象表明 PCB-tBU-SS-PDI NPs 具有良好的生物安全性，可以用作抗肿瘤药物输送的载体。