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Polygonatum polysaccharide modified montmorillonite/chitosan/glycerophosphate composite hydrogel for bone tissue engineering
International Journal of Polymeric Materials and Polymeric Biomaterials ( IF 2.5 ) Pub Date : 2021-08-24 , DOI: 10.1080/00914037.2021.1960336
Xiaoliang Zhao 1 , Pingping Li 1 , Jianning Zhu 2 , Yunya Xia 3 , Jianzhong Ma 1 , Xiuying Pu 1 , Yonggang Wang 1 , Feifan Leng 1 , Yanling Wang 1 , Shuhong Yang 1 , Fen Ran 4 , Dan Tang 1 , Weijie Zhang 1
Affiliation  

Abstract

The stiffness and gelation temperature of thermo-responsive chitosan (CS), glycerophosphate (GP) hydrogel is not enough for differentiation of BMSCs and injecting use in bone defect repair. To improve these weaknesses, a kind of injectable hydrogel (PMCG) was prepared with Chitosan (CS), glycerophosphate (GP), polygonatum polysaccharide modified montmorillonite (PMMT). The gelation temperature of CS/GP (CG) hydrogel was 42 °C, the maximum mechanical strength was 1061 Pa. with the amount of PMMT increased, the gelation temperature decreased to 28 °C, and the maximum stiffness increased to 6789 Pa. At the same time, with the addition of PMMT, the swelling property of hydrogel was improved. The cytocompatibility of PMCG decreased than CG which maybe result from the poor cytocompatibility of montmorillonite, but it was improved with increasing amount of PMMT, which indicated the cell proliferation promoting effect coming from polygonatum polysaccharide. The SEM-EDX exhibited that these hydrogels possessed excellent morphological characteristics and could promote mineralization in simulated body fluid (SBF) solution, PMCG-2 Ca/P has a Ca/P ratio of 1.63 ± 0.04, which is similar to the ratio of human bone mineral (Ca/P ratio of 1.55–1.75). The cell viability confirmed the application availability of the hydrogel. In light of the effect on osteoblasts maturation promoting effect and decrease of osteoclast of polygonatum polysaccharide. We come to the conclusion that it is an effective strategy to improve the mechanic strength and gelation temperature, and keep the osteogenesis promoting effect at the same time.



中文翻译:

黄精多糖改性蒙脱土/壳聚糖/甘油磷酸酯复合水凝胶用于骨组织工程

摘要

热响应壳聚糖(CS)、甘油磷酸(GP)水凝胶的刚度和凝胶温度不足以分化BMSCs和注射用于骨缺损修复。针对这些不足,以壳聚糖(CS)、甘油磷酸盐(GP)、玉竹多糖改性蒙脱土(PMMT)为原料,制备了一种可注射水凝胶(PMCG)。CS/GP(CG)水凝胶的凝胶温度为42℃,最大机械强度为1061 Pa。随着PMMT用量的增加,凝胶温度下降至28℃,最大刚度增加至6789 Pa。同时,随着PMMT的加入,水凝胶的溶胀性能得到改善。PMCG的细胞相容性低于CG,这可能是由于蒙脱石的细胞相容性差,但随着PMMT用量的增加而改善,说明黄精多糖具有促进细胞增殖的作用。SEM-EDX 表明这些水凝胶具有优异的形态特征,在模拟体液 (SBF) 溶液中能促进矿化,PMCG-2 Ca/P 的 Ca/P 比为 1.63 ± 0.04,与人体的比值相似。骨矿物质(Ca/P 比为 1.55–1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。这表明玉竹多糖具有促进细胞增殖的作用。SEM-EDX 表明这些水凝胶具有优异的形态特征,在模拟体液 (SBF) 溶液中能促进矿化,PMCG-2 Ca/P 的 Ca/P 比为 1.63 ± 0.04,与人体的比值相似。骨矿物质(Ca/P 比为 1.55–1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。这表明玉竹多糖具有促进细胞增殖的作用。SEM-EDX 表明这些水凝胶具有优异的形态特征,在模拟体液 (SBF) 溶液中能促进矿化,PMCG-2 Ca/P 的 Ca/P 比为 1.63 ± 0.04,与人体的比值相似。骨矿物质(Ca/P 比为 1.55–1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。SEM-EDX 表明这些水凝胶具有优异的形态特征,在模拟体液 (SBF) 溶液中能促进矿化,PMCG-2 Ca/P 的 Ca/P 比为 1.63 ± 0.04,与人体的比值相似。骨矿物质(Ca/P 比为 1.55–1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。SEM-EDX 表明这些水凝胶具有优异的形态特征,在模拟体液 (SBF) 溶液中能促进矿化,PMCG-2 Ca/P 的 Ca/P 比为 1.63 ± 0.04,与人体的比值相似。骨矿物质(Ca/P 比为 1.55–1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。这与人体骨矿物质的比率相似(Ca/P 比率为 1.55-1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。这与人体骨矿物质的比率相似(Ca/P 比率为 1.55-1.75)。细胞活力证实了水凝胶的应用可用性。鉴于黄精多糖对成骨细胞成熟促进作用和破骨细胞减少的作用。我们得出的结论是,提高机械强度和凝胶温度,同时保持成骨促进作用是一种有效的策略。

更新日期:2021-08-24
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