ABSTRACT

The perspective of this research was to investigate the antioxidant, antibacterial, cytotoxic activity and anti-glycation of the SNPs synthesized by aqueous flower extract of pink Rosa damascena plant and compare with the biological effect of plant extract. The antioxidant activity of plant extract, and SNPs, their inhibitory effect on albumin glycosylation and also the cytotoxic effect on the human fibroblast cells were assessed by DPPH, spectrophotometric assay and MTT test, respectively. The antibacterial activity of SNPs against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumoniae was examined by the disk diffusion method. The percentage of free radicals inhibited by the extract was increased compared to the SNPs. The flower extract and the SNPs both showed anti-glycation effects under in vitro conditions, but SNPs showed the efficient inhibitory effect of on glycation of BSA in the order; 1000 extract < 500 SNP < 250 SNP < 1000 SNP. The toxicity effects of the extract and SNPs were also found to depend on their concentration and time of incubation, still, SNPs showed 8% higher toxicity than the extract. Although SNPs showed antibacterial activity, their effect on S. aureus was significant. It is concluded that the SNPs exhibited multifunctional properties for many medicinal applications.

摘要

本研究的目的是研究粉红蔷薇植物水提取物合成的SNPs的抗氧化、抗菌、细胞毒活性和抗糖化活性，并与植物提取物的生物学效应进行比较。分别通过DPPH、分光光度法和MTT试验评估了植物提取物和SNP的抗氧化活性、它们对白蛋白糖基化的抑制作用以及对人成纤维细胞的细胞毒作用。SNPs对金黄色葡萄球菌、蜡样芽孢杆菌、大肠杆菌和肺炎克雷伯菌的抗菌活性用圆盘扩散法检测。与 SNP 相比，提取物抑制的自由基百分比增加。花提取物和SNPs在体外条件下均表现出抗糖化作用，但SNPs依次显示出对BSA糖化的有效抑制作用；1000 个提取物 < 500 SNP < 250 SNP < 1000 SNP。还发现提取物和 SNP 的毒性作用取决于它们的浓度和孵育时间，但 SNP 的毒性仍比提取物高 8%。尽管 SNP 显示出抗菌活性，但它们对金黄色葡萄球菌的影响是显着的。得出的结论是，SNP 在许多医学应用中表现出多功能特性。